Autophagy biomarkers in CSF correlates with infarct size, clinical severity and neurological outcome in AIS patients.
Autophagy biomarkers in CSF correlates with infarct size, clinical severity and neurological outcome in AIS patients.
复制标题
脑脊液中的自噬生物标志物与 AIS 患者的梗死面积、临床严重程度和神经系统结果相关。
DOI：
10.1186/s12967-015-0726-3
复制
发表时间：
2015-11-14
影响因子：
7.4
通讯作者：
Peng Y
中科院分区：
文献类型：
--
作者：
Li H;Qiu S;Li X;Li M;Peng Y
自噬被证明参与急性缺血性卒中（AIS），然而，这仅限于细胞和/或动物水平。本研究的目的是检测AIS患者脑脊液（CSF）和血清中Beclin1和LC3B两种自噬生物标志物，并评估Beclin1和LC3B水平与脑卒中患者神经功能缺损严重程度和临床转归之间的可能相关性。采用ELISA法测定37例AIS患者和21例对照者脑脊液和血清中Beclin1和LC3B的水平。卒中发作时临床严重程度采用美国国立卫生研究院卒中量表（NIHSS），神经学预后采用改良Rankin量表（mRs）和卒中发作至3个月后NIHSS的改善情况。使用Pearson分析评估自噬生物标志物与梗死体积、NIHSS和mRs之间的关系。与对照组相比，AIS患者CSF和血清中Beclin1和LC3B水平均升高。在脑脊液中，它们与梗死体积和NIHSS评分呈正相关，与mRs评分呈负相关，而在血清中无显著相关性。此外，脑脊液中Beclin1和LC3B水平较高的AIS患者NIHSS的改善程度明显更高。AIS患者CSF和血清自噬生物标志物水平发生改变。脑脊液自噬生物标志物水平与梗死体积、临床严重程度和神经预后相关。
Autophagy is demonstrated to be involved in acute ischemic stroke(AIS), which, however, is confined to cells and/or animals levels. The aim of this study was to determine two autophagy biomarkers, Beclin1 and LC3B, in cerebrospinal fluid (CSF) and serum of patients with AIS, and to evaluate a possible correlation between levels of Beclin1 and LC3B and severity of neurological deficit and clinical outcome of stroke patients. Levels of Beclin1 and LC3B were quantified by ELISA in CSF and serum collected from 37 AIS patients and 21 controls. The clinical severity at stroke onset was determined by the National Institute of Health Stroke Scale (NIHSS) and the neurological outcome was determined by the Modified Rankin Scale (mRs) and the improvement in NIHSS between stroke onset and 3 months later. Associations between autophagy biomarkers and infarct volume, NIHSS and mRs were assessed using Pearson analysis. The levels of Beclin1 and LC3B were increased both in CSF and serum of AIS patients relative to controls. In CSF, they were positively correlated with infarct volume and NIHSS scores, and negatively correlated with mRs scores, but no significant association was observed in serum. Moreover, AIS patients with higher levels of Beclin1 and LC3B in CSF had significantly higher improvement in NIHSS. CSF and serum levels of autophagy biomarkers are altered in AIS patients. CSF levels of autophagy biomarkers are associated with infarct volume, clinical severity of and neurological outcome.