Three-dimensional solution structure of plastocyanin from the green alga Scenedesmus obliquus.
Three-dimensional solution structure of plastocyanin from the green alga Scenedesmus obliquus.
复制标题
绿藻斜生栅藻质体蓝素的三维溶液结构。
DOI：
10.1126/science.3353725
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Wright,PE
中科院分区：
文献类型：
--
作者：
Moore,JM;Case,DA;Chazin,WJ;Gippert,GP;Havel,TF;Powls,R;Wright,PE
利用核磁共振（NMR）技术，通过距离和二面角的限制，确定了绿色藻栅藻（Scenedesmus）质体蓝素的溶液构象。结构生成与距离几何和约束分子动力学计算。一种新的分子置换方法也被用来与相同的NMR约束生成的解决方案结构的S。蓝栅藻质体蓝蛋白的X射线结构与白杨蛋白同源，蓝栅藻质体蓝蛋白在溶液中呈β-桶结构。骨架构象是明确定义的，总体上类似于结晶状态下的白杨质体蓝素。高等植物质体蓝素的独特酸性区域，作为电子转移蛋白和无机复合物的结合位点，在S. aerobusplastocyanin中的形状和电荷都不同。
The solution conformation of plastocyanin from the green algaScenedesmus obliquushas been determined from distance and dihedral angle constraints derived by nuclear magnetic resonance (NMR) spectroscopy. Structures were generated with distance geometry and restrained molecular dynamics calculations. A novel molecular replacement method was also used with the same NMR constraints to generate solution structures ofS. obliquusplastocyanin from the x-ray structure of the homologous poplar protein.Scenedesmus obliquusplastocyanin in solution adopts a β-barrel structure. The backbone conformation is well defined and is similar overall to that of poplar plastocyanin in the crystalline state. The distinctive acidic region of the higher plant plastocyanins, which functions as a binding site for electron transfer proteins and inorganic complexes, differs in both shape and charge inS. obliquusplastocyanin.