MICROSATELLITE MARKERS IN THE TREE PEONY, PAEONIA SUFFRUTICOSA (PAEONIACEAE)
MICROSATELLITE MARKERS IN THE TREE PEONY, PAEONIA SUFFRUTICOSA (PAEONIACEAE)
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DOI：
10.3732/ajb.1000127
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Fluch, Silvia
中科院分区：
文献类型：
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作者：
Homolka, Andreas;Berenyi, Maria;Fluch, Silvia
本研究的目的：方法和结果：利用SSR富集文库和EST-mining技术，从奥地利牡丹品种中筛选出8对多态性引物。引物扩增的二核苷酸重复序列和三核苷酸重复序列每个位点有2-6个等位基因。所有引物在口氏原海葵、庞氏原海葵变种中均能扩增出条带。结论：这些结果显示了引物在P. suffruticosa中用于群体遗传研究的有用性以及它们在跨属相关类群中交叉扩增的能力。
Premise of the study: Microsatellite primers were developed in the tree peony, Paeonia suffruticosa, to perform paternity tests as well as assignment to variety in special Austrian collections.Methods and Results: Using SSR-enriched libraries and EST-mining, 8 polymorphic primer sets were identified in Austrian collections of Paeonia sect. Moutan DC. The primers amplified di- and trinucleotide repeats with 2-6 alleles per locus. All primers also amplified in P. ostii, P. pontaninii var. trolloides, P. delavayi, and P. lutea, and in the herbaceous species P. peregrina and P. tenuifolia (Paeonia sect. Paeon).Conclusions: These results show the usefulness of primers in P. suffruticosa for population genetic studies and their ability to cross amplify in related taxa across the genus.