High-dose ascorbic acid administration improves functional recovery in rats with spinal cord contusion injury
High-dose ascorbic acid administration improves functional recovery in rats with spinal cord contusion injury
复制标题
DOI：
10.1038/sc.2014.135
复制
发表时间：
2014-09
期刊：
影响因子：
2.2
通讯作者：
M. Yan;M. Yang;W. Shao;X-g Mao;B. Yuan;Y-f Chen;Z-x Ye;W. Liang;Z-j Luo
中科院分区：
文献类型：
--
作者：
M. Yan;M. Yang;W. Shao;X-g Mao;B. Yuan;Y-f Chen;Z-x Ye;W. Liang;Z-j Luo
目的：探讨不同剂量抗坏血酸(AA)对标准脊髓损伤(SCI)大鼠功能表现的影响。方法：雌性SD大鼠30只，随机分为3组，每组10只：对照组：脊髓损伤后给予生理盐水；正常剂量组：脊髓损伤后每日给予抗坏血酸(AA)100 mg/kg−-1体重；大剂量抗坏血酸(AA)组：脊髓损伤后每日给予AA 200 mg/kg−-1体重。采用Basso、Beattie、Bresna han运动功能评分(BBB评分)和足迹分析评价各组大鼠的功能状态，苏木精-伊红染色评价损伤部位的坏死情况。结果：脊髓损伤后第14天和第28天，AA高剂量组大鼠的BBB评分明显好于对照组(P<；0.05)，而中剂量AA组大鼠的BBB评分与对照组无显著差异。与对照组和中剂量AA组相比，AA高剂量组大鼠的步长增加，步宽减小，脚趾拖曳减少(P<；0.05)。组织学分析显示，与对照组相比，正常剂量和高剂量AA组损伤部位的坏死均明显减少(P<；0.05)。结论：大剂量AA在大鼠脊髓损伤后急性期应用可显著减少继发性损伤引起的组织坏死，改善大鼠的功能表现。
Objectives:To evaluate the effects of different doses of ascorbic acid (AA) on the functional performance of rats subjected to standardized spinal cord injury (SCI).Methods:Thirty female Sprague–Dawley rats were divided into three groups (10 animals in each group): control group: rats were subjected to SCI injury and received intraperitoneal saline administration; normal-dose AA group: rats were subjected to SCI injury and received daily intraperitoneal administration of AA at 100 mg kg− 1 bodyweight; high-dose AA group: rats were subjected to SCI injury and received daily intraperitoneal administration of AA at 200 mg kg− 1 bodyweight. The Basso, Beattie, Bresnahan Locomotor Rating Score (BBB score) and footprint analysis were performed to evaluate the functional performance of the rats in each group, and hematoxylin and eosin staining was performed to evaluate necrosis at the injury site.Results:At days 14 and 28 after SCI, rats in the high-dose AA group, but not the normal-dose AA group, exhibited significantly better BBB score compared with the control group (P< 0.05). Compared with the control and normal-dose AA group, the high-dose AA group also showed increased stride length, decreased stride width and reduced toe dragging (P< 0.05). Histological analysis revealed that both the normal-and high-dose AA groups had reduced necrosis in the injury site compared with the control group (P< 0.05).Conclusion:High-dose AA administration during the acute phase post SCI significantly reduced secondary injury-induced tissue necrosis and improved functional performance in rats.