Amiloride directly inhibits the Na,K-ATPase activity of rabbit kidney proximal tubules.
Amiloride directly inhibits the Na,K-ATPase activity of rabbit kidney proximal tubules.
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阿米洛利直接抑制兔肾近曲小管的 Na,K-ATP 酶活性。
DOI：
10.1126/science.6302840
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Mandel,LJ
中科院分区：
文献类型：
--
作者：
Soltoff,SP;Mandel,LJ
阿米洛利抑制哇巴因敏感的氧消耗率（QO 2）的悬浮液中的兔完整的近端小管在不同浓度的细胞外钠的存在下。在制霉菌素存在的情况下，测量哇巴因敏感的QO 2，悬浮液中小管的组织钠和钾含量，以及溶解的小管膜的钠和钾依赖性腺苷三磷酸酶（Na，K-ATP酶）活性，表明阿米洛利的作用是由于直接抑制近端小管的Na，K-ATP酶活性。
Amiloride inhibited the ouabain-sensitive rate of oxygen consumption (QO2) of a suspension of rabbit intact proximal tubules in the presence of different concentrations of extracellular sodium. Measurements of the ouabain-sensitiveQO2in the presence of nystatin, the tissue sodium and potassium contents of the tubules in suspension, and the sodium- and potassium-dependent adenosinetriphosphatase (Na,K-ATPase) activity of lysed tubule membranes indicated that the effect of amiloride was due to a direct inhibition of the Na,K-ATPase activity of the proximal tubule.