The novel untranslated exon "exon 0T" encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha (ER alpha) gene.
The novel untranslated exon "exon 0T" encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha (ER alpha) gene.
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新的非翻译外显子“外显子0T”编码在大鼠雌激素受体α（ERα）基因的外显子0和外显子1之间。
DOI：
10.1507/endocrj.48.465
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发表时间：
2001
影响因子：
2
通讯作者：
K. Hoshi
中科院分区：
文献类型：
--
作者：
N. Osada;S. Hirata;T. Shoda;K. Hoshi
我们最近利用cDNA末端5'-快速扩增(5'-RACE)方法从肝脏中分离出大鼠雌激素受体α(ERα)基因的两个非翻译第一外显子，外显子0N和外显子0S。在本次交流中，我们进一步分析了大鼠垂体前部ERα mRNA的5'-非翻译区（UTR），以研究大鼠ERα基因的其他5'-非翻译外显子的存在。使用位于大鼠 ER α 基因外显子 1 的反义引物对来自 8 周龄雌性 Wistar 品系雄性大鼠前垂体的总 RNA 进行 5'-RACE，并对阳性克隆之一（克隆 35）进行测序。克隆35的核苷酸序列揭示了在外显子0（第一个报道的大鼠ERαmRNA的5'-UTR形式）和大鼠ERαmRNA的外显子1之间插入了先前未识别的外显子（我们将其称为“外显子0T”）。大鼠基因组DNA分析表明，外显子0T位于大鼠ERα基因的外显子0和外显子1之间。我们通过逆转录聚合酶链式反应（RT-PCR）进一步研究了含有外显子0T的ERαmRNA在8周龄雄性和雌性Wistar品系大鼠的几个脑区和各种外周组织中的分布。 ER α mRNA (0T-1) 的分布与之前报道的 ER α mRNA 的外显子 0 剪接到外显子 1 上的分布基本相似。这些结果表明(1)外显子0T是大鼠ERα基因的一个新的非翻译外显子，位于大鼠基因组DNA上的外显子0和外显子1之间，(2)外显子0T通过选择性剪接插入ERαmRNA的外显子0和外显子1之间，(3)这种选择性剪接可能发生在ERα基因转录从外显子0开始的组织中。
We have recently isolated two untranslated first exons, exon 0N and exon 0S, of rat estrogen receptor alpha (ER alpha) gene from the liver by use of 5'-rapid amplification of the cDNA ends (5'-RACE) method. In this communication, we further analyzed the 5'-untranslated region (UTR) of the ER alpha mRNA in rat anterior hypophysis in order to investigate the existence of the other 5'-untranslated exon(s) of rat ER alpha gene. Total RNA from the anterior hypophysis of 8-week-old female Wistar strain male rats was subjected to 5'-RACE with antisense primers located in exon 1 of the rat ER alpha gene and one of the positive clones (clone 35) was sequenced. The nucleotide sequence of clone 35 revealed the insertion of a previously unidentified exon (which we termed "exon 0T") between exon 0 (the first reported 5'-UTR form of rat ER alpha mRNA) and exon 1 of rat ER alpha mRNA. Analysis of rat genomic DNA indicated that exon 0T was located between exon 0 and exon 1 of rat ER alpha gene. We further investigated the distribution of ER alpha mRNA containing exon 0T in several brain regions and various peripheral tissues of 8-week-old male and female Wistar strain rats by use of reverse transcription-polymerase chain reaction (RT-PCR). The distribution of the ER alpha mRNA (0T-1) was essentially similar to that of ER alpha mRNA in which exon 0 was spliced onto exon 1 reported previously. These results indicate that (1) exon 0T is a novel untranslated exon of rat ER alpha gene which is located between exon 0 and exon 1 on rat genomic DNA, (2) exon 0T is inserted between exon 0 and exon 1 of ER alpha mRNA by alternative splicing, and (3) this alternative splicing may occur in tissues where the transcription of ER alpha gene is initiated from exon 0.
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR