Genome-wide expression analysis suggests a role for jasmonates in the resistance to blue mold in apple
Genome-wide expression analysis suggests a role for jasmonates in the resistance to blue mold in apple
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DOI：
10.1007/s10725-018-0388-2
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发表时间：
2018-07-01
影响因子：
4.2
通讯作者：
Renou, Jean-Pierre
中科院分区：
文献类型：
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作者：
Ahmadi-Afzadi, Masoud;Orsel, Mathilde;Renou, Jean-Pierre
苹果蓝霉病是由坏死营养型病原菌扩展青霉（Penicilliumexpansum）引起的，其产生的棒曲霉素（patulin）严重威胁着人类的健康。最近的研究表明，苹果品种对这种疾病的抗性是定量控制的。使用覆盖60k转录物的全基因组苹果微阵列来鉴定在扩展疫霉感染的果实中似乎在抗性和感病品种之间差异调节的基因。在防御和氧化应激相关基因、细胞壁修饰和木质化基因以及与定位和运输相关的基因中，遇到了一些潜在的候选基因。诱导一个细胞壁相关基因和三个参与“下游”类黄酮生物合成途径的基因，表明细胞壁作为一个重要屏障的基本作用，并表明水果类黄酮参与抗蓝霉菌。此外，植物激素茉莉酸甲酯（MeJA）的外源性应用减少了由红苹果与扩展疫霉引起的症状。这是第一份将MeJA和细胞壁和类黄酮途径基因的激活与抗蓝霉病联系起来的报告，比较了不同品种的驯化苹果。我们的研究结果提供了一个初步分类的基因，可能参与的抗性机制，并应是有用的开发工具，基因标记辅助育种的苹果品种具有改善的抗白粉病。
Blue mold, caused by the necrotrophic fungal pathogen Penicillium expansum, causes serious postharvest losses in apple, and threatens human health through production of the potent mycotoxin patulin. Recent studies indicate a quantitative control of resistance against this disease in apple cultivars. A whole genome apple microarray covering 60k transcripts was used to identify gene(s) that appear to be differentially regulated between resistant and susceptible cultivars in P. expansum-infected fruits. A number of potential candidates was encountered among defense- and oxidative stress-related genes, cell wall modification and lignification genes, and genes related to localization and transport. Induction of one cell wall-related gene and three genes involved in the 'down-stream' flavonoid biosynthesis pathway, demonstrates the fundamental role of the cell wall as an important barrier, and suggests that fruit flavonoids are involved in the resistance to blue mold. Moreover, exogenous application of the plant hormone methyl jasmonate (MeJA) reduced the symptoms resulting from inoculating apples with P. expansum. This is the first report linking MeJA and activation of cell wall and flavonoid pathway genes to resistance against blue mold in a study comparing different cultivars of domesticated apple. Our results provide an initial categorization of genes that are potentially involved in the resistance mechanism, and should be useful for developing tools for gene marker-assisted breeding of apple cultivars with an improved resistance to blue mold.