The MdAP2-34 modulates flavonoid accumulation in apple (Malus domestica Borkh.) by regulating MdF3′H
The MdAP2-34 modulates flavonoid accumulation in apple (Malus domestica Borkh.) by regulating MdF3′H
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DOI：
10.1016/j.postharvbio.2022.111994
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发表时间：
2022-06-06
影响因子：
7
通讯作者：
Jia, Dongjie
中科院分区：
文献类型：
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作者：
Han, Dong;Huang, Benchang;Jia, Dongjie
黄酮类化合物是园艺水果中的主要色素之一。AP2/ERF转录因子在高等植物中发挥着多种作用，其中MdAP2-34调控苹果类胡萝卜素的积累。然而，目前尚不清楚光线是否会干扰苹果果肉中类黄酮的积累。本研究发现，光照处理下的“贝宁将军”苹果果肉黄酮类化合物含量高于光照处理，收获后15天（DAH）和30天（DAH）分别增加了48%和39%。光照和暗处理下MdAP2-34转录本水平与“贝宁将军”果实类黄酮含量呈高度正相关。MdAP2-34-OVX苹果愈伤组织中，黄酮醇生物合成结构基因MdCHS、MdCHI、mdf3’h和MdFLS与果实类黄酮含量呈正相关，且表达上调。MdAP2-34通过直接结合并激活mdf3’h启动子活性，通过类黄酮生物合成途径刺激类黄酮积累。我们的研究结果表明，利用mdap2 -34介导的多种功能来理解苹果类黄酮合成的复杂机制，这也可能对苹果育种有用。
Flavonoids are one of the main pigments in horticultural fruit. The AP2/ERF transcription factors play various roles in higher plants, and thus, MdAP2-34 has been reported to regulate carotenoid accumulation in apple. However, it is unclear whether light interferes with the flavonoid accumulation in apple fruit flesh. Here, we found that apple of 'Benin Shogun' fruit flesh had higher levels of flavonoids under light treatment than under dark treatment, with an increase of 48% at 15 days after harvest (DAH) and 39% at 30 DAH. The MdAP2-34 transcript levels exhibited a highly positive correlation with the flavonoid content of 'Benin Shogun' fruit under dark and light treatments. Furthermore, the structural genes of flavonol biosynthesis, including MdCHS, MdCHI, MdF3'H, and MdFLS, were positively associated with flavonoid contents of fruit and were upregulated in MdAP2-34-OVX apple calli. The MdAP2-34 stimulated flavonoid accumulation via the flavonoid biosynthetic pathway by directly binding to and activating the MdF3'H promoter activity. Our findings demonstrate the use of multiple MdAP2-34-mediated functions in understanding the complex mechanisms of flavonoid synthesis in apple, which may also be useful in apple breeding.