Systematic evaluation of biocompatibility of magnetic Fe3O4 nanoparticles with six different mammalian cell lines
Systematic evaluation of biocompatibility of magnetic Fe3O4 nanoparticles with six different mammalian cell lines
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DOI：
10.1007/s11051-010-0019-y
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发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
Wang, Jinke
中科院分区：
文献类型：
--
作者：
Liu, Yingxun;Chen, Zhongping;Wang, Jinke
本文系统地评估了多种哺乳动物细胞系与 11 nm DMSA 包被的 Fe3O4 磁性纳米颗粒 (MNP) 的生物相容性。将RAW264.7、THP-1、Hepa1-6、HepG2、HL-7702和HeLa细胞与六种不同浓度（0、20、30、40、50和100μg/mL）的MNP孵育48小时，然后定量评估细胞标记、铁负载、细胞活力、凋亡、周期和氧化应激。结果表明，所有细胞均被纳米颗粒有效标记；然而，在任何剂量下，RAW264.7 的铁载量均显着高于其他细胞。除暴露于100μg/mL MNP的HepG2外，所有细胞的增殖均未受到所研究剂量的MNP的显着抑制。氧化应激研究表明，所有剂量MNP处理的细胞中总超氧化物歧化酶和黄嘌呤氧化酶水平均无明显变化，而MNP处理细胞的丙二醛活性水平显着升高。纳米颗粒在任何剂量下均未对细胞周期产生任何显着影响，但在最高浓度100μg/mL时导致THP-1和HepG2细胞显着凋亡。在人体研究中使用浓度为 30 μg/mL 的血管内纳米颗粒成像剂（Combidex）时，纳米颗粒有效地标记了所有研究的细胞，但不会对其活力、氧化应激、细胞凋亡和周期产生任何显着影响。因此，该纳米颗粒具有较好的生物相容性，为其临床应用提供了一些有用的信息。
This article systematically evaluated the biocompatibility of multiple mammalian cell lines to 11-nm DMSA-coated Fe3O4 magnetic nanoparticles (MNPs). Cells including RAW264.7, THP-1, Hepa1-6, HepG2, HL-7702, and HeLa were incubated with six different concentrations (0, 20, 30, 40, 50, and 100 mu g/mL) of MNPs for 48 h, and then the cell labeling, iron loading, cell viability, apoptosis, cycle, and oxidative stress were all quantitatively evaluated. The results revealed that all the cells were effectively labeled by the nanoparticles; however, the iron loading of RAW264.7 was significantly higher than that of other cells at any dose. The proliferations of all the cells were not significantly suppressed by MNPs at the studied dose except HepG2 that was exposed to 100 mu g/mL MNPs. The investigation of oxidative stress demonstrated that the levels of total superoxide dismutase and xanthine oxidase had no significant changes in all the cells treated by all the doses of MNPs, while the levels of malonyldialdehyde activity of MNP-treated cells significantly increased. The nanoparticles did not produce any significant effect on cell cycles at any of the doses, but resulted in significant apoptosis of THP-1 and HepG2 cells at the highest concentration of 100 mu g/mL. At a concentration of 30 mu g/mL which was used in human studies with an intravascular nanoparticle imaging agent (Combidex), the nanoparticles efficiently labeled all the cells studied, but did not produce any significant influence on their viability, oxidative stress, and apoptosis and cycle. Therefore, the nanoparticles were concluded with better biocompatibility, which provided some useful information for its clinical applications.