Optogenetic control of heart muscle in vitro and in vivo
Optogenetic control of heart muscle in vitro and in vivo
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DOI：
10.1038/nmeth.1512
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发表时间：
2010-11-01
期刊：
影响因子：
48
通讯作者：
Sasse, Philipp
中科院分区：
文献类型：
--
作者：
Bruegmann, Tobias;Malan, Daniela;Sasse, Philipp
电刺激是探索心肌电气行为的标准技术，但是这种方法具有相当大的技术限制。在这里，我们报告了光激活的阳离子通道通道Ropopsin-2的表达，用于在体外和小鼠中对心肌刺激的光诱导。这种方法实现了心肌细胞和心脏组织的精确局部刺激，并持续长时间的去极化，从而改变了起搏器的改变，CA（2+）稳态，电耦合和心律失常的自发性外部额外额外额外的外部。
Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca(2+) homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.