Utility of morphological abnormalities during early-life development of the estuarine mummichog, Fundulus heteroclitus, as an indicator of estrogenic and antiestrogenic endocrine disruption
Utility of morphological abnormalities during early-life development of the estuarine mummichog, Fundulus heteroclitus, as an indicator of estrogenic and antiestrogenic endocrine disruption
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DOI：
10.1897/03-50
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发表时间：
2004-02-01
影响因子：
4.1
通讯作者：
van der Kraak, GJ
中科院分区：
文献类型：
--
作者：
Boudreau, M;Courtenay, SC;van der Kraak, GJ
为了评估形态异常用于内分泌干扰物质(EDS)标准测试的使用，我们测试了一种假设，即发育异常是常见河口青鱼(双鱼科：鲤科)胚胎、幼体和幼体阶段暴露于水中雌激素和抗雌激素EDS的敏感指标。每天更新10~10,000 ng/L的雌激素激动剂17α-乙炔雌二醇(EE2)或拮抗剂ZM189,154(ZM)，在生命的前25或60天进行静态暴露。雌二醇显著增加骨骼异常(脊柱侧弯、前凸、头部、颅面、颌骨、鳍)和软组织异常(肛门肿胀)的发生率，但仅在高浓度(1,000或10,000 ng/L)时才显著增加。暴露60天比暴露25天产生了更严重的异常，鱼的比例也更高。在较长的暴露时间内，10,000 ng/L的EE2产生的异常鱼比1,000 ng/L的多(100%比51.6%)，每条异常鱼的异常鱼数(5.73比1.47)。在清洁水中饲养至12个月的鱼，在暴露于10,000 ng/L的EE2 60d后，成活率低于对照组，除肛门肿胀外，仍保留异常，与暴露于其他浓度的EE2和ZM的鱼一样，在6个月和12个月时体重增加。接触ZM 60天会增加脊柱侧弯的发生率(1,000 ng/L)，但降低异常鱼类和脊柱前凸的总体发生率(10和10,000 ng/L)。孵化前未观察到EE2或ZM的影响，幼虫的清除和染色表明，脊椎异常的表达与骨化在时间上是一致的。我们的结论是，木乃伊猪的形态异常不是环境中暴露于雌激素或抗雌激素水性EDSS的敏感指标。相关浓度。
To evaluate the use of morphological abnormalities for standard testing of endocrine-disrupting substances (EDS), we tested the hypothesis that developmental abnormalities are a sensitive indicator of exposure to waterborne estrogenic and antiestrogenic EDS during embryonic, larval, and juvenile stages in the common estuarine killifish, the mummichog (Pisces: Cyprinodontidae). Static exposures with daily renewal were carried out with 10 to 10,000 ng/L of the estrogen agonist 17alpha-ethynylestradiol (EE2) or antagonist ZM189,154 (ZM) for the first 25 or 60 d of life. Incidence of skeletal abnormalities (scoliosis, lordosis, head, craniofacial, jaw, fin) and soft tissue abnormality (anal swelling) were significantly increased by EE2 but only at high concentrations (1,000 or 10,000 ng/L). Sixty-day exposure produced more severe abnormalities than 25-d exposure and in a higher proportion of fish. Within the longer exposure, 10,000 ng/L EE2 produced more abnormal fish than 1,000 ng/L (100% vs 51.6%) and more abnormalities per abnormal fish (5.73 vs 1.47). Fish reared to 12 months in clean water after exposure for 60 d to 10,000 ng/L EE2 survived at a lower rate than controls, retained abnormalities with the exception of anal swelling and, like fish exposed to other concentrations of EE2 and ZM, showed increased weight at length at 6 and 12 months. Sixty-day exposure to ZM increased the incidence of scoliosis (1,000 ng/L) but decreased the overall incidences of abnormal fish and lordosis (10 and 10,000 ng/L). No impacts of EE2 or ZM were observed before hatch, and clearing and staining of larvae demonstrated that expression of vertebral abnormalities coincided temporally with ossification. We conclude that morphological abnormalities in mummichogs are not a sensitive indicator of exposure to estrogenic or antiestrogenic waterborne EDSs at environmentally. relevant concentrations.