Inner limiting membrane as membranous support in RPE sheet-transplantation
Inner limiting membrane as membranous support in RPE sheet-transplantation
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DOI：
10.1007/s00417-007-0566-9
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发表时间：
2007-10-01
影响因子：
2.7
通讯作者：
Grisanti, Salvatore
中科院分区：
文献类型：
--
作者：
Beutel, Julia;Greulich, Lena;Grisanti, Salvatore
目的探讨内界膜作为视网膜色素上皮细胞（retinal pigment epithelial cells，RPE）支架的作用。这些人供体眼睛和另外的猪眼睛（n=11）用于分离RPE细胞。使用人RPE细胞系（ARPE-19）作为对照。将RPE细胞在ILM上培养3天和7天。获得培养中细胞的相差照片。光镜和透射电镜观察细胞形态和超微结构的变化。细胞保持其立方形形态，被极化，在顶面上显示微绒毛，形成细胞间连接，并且不去分化。从尸体眼获得的人RPE细胞几乎不粘附于ILM，并且不形成完整的单层。ARPE-19细胞集落密度高，细胞形态完整。结论ILM是建立完整RPE单层的理想基质，可作为视网膜下移植的材料。
Purpose To investigate the inner limiting membrane as a scaffold for retinal pigment epithelium cells (RPE).Methods Human donor eyes (n=10) from the Eye Bank Tubingen were used to collect the inner limiting membrane (ILM). These human donor eyes and additional porcine eyes (n=11) served to isolate RPE cells. A human RPE cell line (ARPE-19) was used as control. RPE cells were cultured on ILM for 3 and 7 days. Phase-contrast photographs of the cells in culture were obtained. Morphology and ultrastructural changes were evaluated by light and transmission electron microscopy.Results Porcine RPE cells adhere and proliferate when seeded on human ILM. The cells maintained their cuboidal morphology, were polarized, disclosed microvilli on the apical surface, formed intercellular junctions and did not dedifferentiate. Human RPE cells obtained from cadaver eyes barely adhered to the ILM and did not form an intact monolayer. ARPE-19 cells formed a dense colony and maintained epithelial features.Conclusion The ILM is an ideal matrix to establish an intact RPE monolayer and has the potential to be used as sheet for subretinal transplantation.