PERIPHERIN AND NEUROFILAMENT PROTEIN COEXIST IN SPINAL SPHEROIDS OF MOTOR-NEURON DISEASE
PERIPHERIN AND NEUROFILAMENT PROTEIN COEXIST IN SPINAL SPHEROIDS OF MOTOR-NEURON DISEASE
复制标题
DOI：
10.1097/00005072-199209000-00008
复制
发表时间：
1992-09-01
影响因子：
3.2
通讯作者：
HAYS, AP
中科院分区：
文献类型：
--
作者：
CORBO, M;HAYS, AP
我们比较了神经丝蛋白（NF）与其他两种神经元特异性中间丝蛋白在运动神经元疾病和对照组的大脊髓轴突肿胀（球体）中的免疫定位。所有球体分别标记NF的三个不同亚基，即低分子量、中分子量和高分子量多肽。双标记免疫荧光法对300个轴突肿胀进行NF免疫染色，其中87%含有中间蛋白外周蛋白。在共聚焦显微镜下，在1 μ m光学切片的高分辨率下，NF和外周蛋白的免疫染色模式难以区分。少数球状体含有另一种中间蛋白α -连接蛋白，但其免疫反应性较弱。在运动神经元疾病和对照组中检测的所有表位轴突肿胀的免疫染色是相似的。结果表明，外周蛋白和神经丝蛋白是球体中增殖的中间丝的主要成分。
We have compared the immunolocalization of neurofilament protein (NF) with two other neuronal-specific intermediate filament proteins in large spinal axonal swellings (spheroids) of motor neuron disease and controls. All spheroids labeled each of the three different subunits of NF, the low, middle, and high molecular weight polypeptides. In doublelabel immunofluorescence, 300 axonal swellings were immunostained for NF, and 87% of them contained the intermediate protein peripherin. The pattern of immunostaining of NF and peripherin was indistinguishable at a high resolution viewed in 1-mu-m optical sections by confocal microscopy. A minority of the spheroids contained alpha-internexin, another intermediate protein, but it was weakly immunoreactive. The immunostaining of axonal swellings was similar for all epitopes tested in motor neuron disease and control subjects. The findings suggest that peripherin as well as neurofilament protein are major components of the proliferated intermediate filaments in spheroids.