Low levels of IgG antibodies against Pneumocystis carinii among HIV-infected patients
Low levels of IgG antibodies against Pneumocystis carinii among HIV-infected patients
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DOI：
10.1080/00365549850161511
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发表时间：
1998-01-01
影响因子：
--
通讯作者：
Andersen, PL
中科院分区：
文献类型：
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作者：
Laursen, AL;Andersen, PL
以人和大鼠的卡氏肺孢子虫(P, carinii)的尿素提取物为抗原，通过ELISA法检测了针对卡氏肺孢子虫(P, carinii)的IgG抗体。碳水化合物构成了 ELISA 测定中负责反应性的抗原的主要部分，因为高碘酸盐处理降低了大多数测试血清的反应性。检测到人和大鼠 P, carinii 之间的交叉反应性。然而，人血清可识别人卡氏肺结核特异性抗原，通过 ELISA 方法比较了献血者 (n = 40)、无症状 HIV 抗体阳性患者 (n = 30) 和患有卡氏肺结核 (PCP) 的艾滋病患者 (n = 22) 和既往没有卡氏肺结核 (PCP) 的艾滋病患者 (n = 21) 之间的 IgG 抗体水平。与献血者相比，艾滋病毒感染者针对微生物的抗体反应性明显较低。在 HIV 抗体阳性患者中，PCP 患者的抗体反应性最高。由于相同数量的患者抗体反应性增加和减少，因此对 PCP 的抗体反应受损。总之，碳水化合物是卡氏疟原虫免疫原性抗原的重要组成部分。大鼠和人 P, carinii 之间的交叉反应性已得到证实，但使用大鼠抗原时反应性稍低。 HIV 感染患者的抗体水平较低，并且对感染产生抗体反应的能力受损，这表明抗体反应较差可能导致 HIV 感染患者容易患 PCP。
IgG antibodies against Pneumocystis carinii (P, carinii) were detected by an ELISA method using urea-extracted material from human and rat P, carinii as the antigen. Carbohydrate formed a major part of the antigen responsible for reactivity in the ELISA assay, since periodate treatment reduced the reactivity of most sera tested. Cross-reactivity between human and rat P, carinii was detected. However, human serum recognized antigens specific for human P, carinii, With the ELISA method IgG antibody levels were compared between blood donors (n = 40), asymptomatic HIV-antibody positive patients (n = 30) and AIDS patients with (n = 22) and without previous P, carinii pneumonia (PCP) (n = 21). HIV-infected patients had significantly lower antibody reactivity against the microorganism compared with blood donors. Among HIV-antibody positive patients the highest antibody reactivity was seen in PCP patients. The antibody response to PCP was impaired, since an equal number of patients had an increase and a decrease in antibody reactivity. In conclusion, carbohydrate formed an important part of the P, carinii immunogenic antigen. Cross-reactivity between rat and human P, carinii was demonstrated, but reactivity was somewhat lower using antigen from rats. The antibody level was lower in HIV-infected patients and the ability to mount an antibody response to the infection was impaired, suggesting that the poor antibody response may contribute to the liability of HIV-infected patients to have PCP.