Overexpression of the homoterpene synthase gene, OsCYP92C21, increases emissions of volatiles mediating tritrophic interactions in rice.
Overexpression of the homoterpene synthase gene, OsCYP92C21, increases emissions of volatiles mediating tritrophic interactions in rice.
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DOI：
10.1111/pce.13924
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发表时间：
2020-10
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通讯作者：
Wei Li;Lingnan Wang;Fei Zhou;Changyan Li;Weihua Ma;Hao Chen;Guirong Wang;J. Pickett;Jing-Jiang Zhou;Yongjun Lin
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作者：
Wei Li;Lingnan Wang;Fei Zhou;Changyan Li;Weihua Ma;Hao Chen;Guirong Wang;J. Pickett;Jing-Jiang Zhou;Yongjun Lin
植物防御高萜类化合物可用于吸引害虫天敌。然而，高萜类化合物在水稻中的生物合成途径仍然是未知的，并且由于其从植物中的低排放，这种间接防御系统的实际应用受到固有的限制。在这里，我们证明了蛋白质OsCYP92C21是负责高萜类生物合成在水稻中。我们还发现，水稻产生高萜类化合物的能力是依赖于亚细胞前体库。通过特异性亚细胞靶向表达、遗传转化和遗传渐渗等方法增加前体库，显著提高了水稻中高萜的生物合成。最终渗入的转基因水稻植物表现出比野生型水稻更高的高萜排放，并且即使没有诱导食草动物攻击，也是迄今为止报道的此类转基因植物的最高萜排放。因此，这些转基因水稻植株对寄生蜂Cotesia chilonis表现出强烈的吸引力。本研究发现了水稻高萜生物合成途径，通过遗传转化增加亚细胞区室前体库和过表达高萜合成酶，为植物间接防御机制在害虫综合治理的“推拉”策略中的利用奠定了基础。本文受版权保护。All rights reserved.
Plant defense homoterpenes can be used to attract pest natural enemies. However, the biosynthetic pathway of homoterpenes is still unknown in rice, and the practical application of such indirect defense systems suffers from inherent limitations due to their low emissions from plants. Here, we demonstrated that the protein OsCYP92C21 is responsible for homoterpene biosynthesis in rice. We also revealed that the ability of rice to produce homoterpenes is dependent on the subcellular precursor pools. By increasing the precursor pools through specifically subcellular targeting expression, genetic transformation and genetic introgression, we significantly enhanced homoterpene biosynthesis in rice. The final introgressed GM rice plants exhibited higher homoterpene emissions than the wild type rice and the highest homoterpene emission reported so far for such GM plants even without the induction of herbivore attack. As a result, these GM rice plants demonstrated strong attractiveness to the parasitic wasp Cotesia chilonis. This study discovered the homoterpene biosynthesis pathway in rice, and lays the foundation for the utilization of plant indirect defense mechanism in the "push-pull" strategy of integrated pest management through increasing precursor pools in the subcellular compartments and overexpressing homoterpene synthase by genetic transformation. This article is protected by copyright. All rights reserved.