A transposon insertion in the gumG homologue of Xanthomonas oryzae pv. oryzae causes loss of extracellular polysaccharide production and virulence
A transposon insertion in the gumG homologue of Xanthomonas oryzae pv. oryzae causes loss of extracellular polysaccharide production and virulence
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DOI：
10.1111/j.1574-6968.1999.tb08707.x
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发表时间：
1999-10-01
影响因子：
2.1
通讯作者：
Sonti, RV
中科院分区：
文献类型：
--
作者：
Dharmapuri, S;Sonti, RV
水稻黄单胞菌（Xanthomonas oryzae pv, oryzae）引起一种严重的水稻疾病，称为细菌性叶枯病。它产生大量的细胞外多糖（EPS）。一个缺乏EPS和毒力的稻瘟病病菌突变体。经Tn5诱变分离得到oryzae。在大肠杆菌载体pBluescript上用转座子标记克隆了该突变等位基因，并测定了转座子插入位点两侧的DNA序列。使用BLAST算法在DNA数据库中基于计算机的相似性搜索显示，这些序列在核苷酸水平上与树胶簇中的一个基因gumG相同，这是黄单胞菌合成EPS所必需的。定。一个36-kb的X. oryzae pv。稻瘟病菌基因组克隆包含稻瘟病菌EPS生物合成基因簇。oryzae恢复了gumGXo::Tn5突变体的EPS产量和毒力能力。结果表明，EPS是水稻弧菌的重要毒力因子。oryzae。(C) 1999年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
Xanthomonas oryzae pv, oryzae causes a serious disease of rice called bacterial leaf blight. It produces copious amounts of extracellular polysaccharide (EPS). An EPS- and virulence-deficient mutant of X. oryzae pv. oryzae was isolated by Tn5 mutagenesis. The mutant allele in this strain was cloned by transposon tagging in the Escherichia coli vector pBluescript and the DNA sequences flanking the transposon insertion site were determined. Computer-based similarity searches in the DNA database using the BLAST algorithm showed these sequences to be 78% identical at the nucleotide level to a gene, gumG, in the gum cluster, which is required for EPS biosynthesis in Xanthomonas campestris pv. campestris. A 36-kb X. oryzae pv. oryzae genomic clone containing the putative EPS biosynthetic gene cluster of X. oryzae pv. oryzae restored both EPS production and virulence proficiency to the gumGXo::Tn5 mutant. The results suggest that EPS is an important virulence factor of X. oryzae pv. oryzae. (C) 1999 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.