Virus-specific thermostability and heat inactivation profiles of alphaviruses
Virus-specific thermostability and heat inactivation profiles of alphaviruses
复制标题
DOI：
10.1016/j.jviromet.2016.04.004
复制
发表时间：
2016-08-01
影响因子：
3.1
通讯作者：
Vanlandingham, Dana L.
中科院分区：
文献类型：
--
作者：
Park, So Lee;Huang, Yan-Jang S.;Vanlandingham, Dana L.
血清学诊断是疾病监测的关键组成部分，对于解决甲病毒（如基孔肯雅病毒（CHIKV）和罗斯河病毒（RRV））发病率和疾病负担的增加非常重要。血清学诊断的金标准是空斑减少中和试验（PRNT），该试验证明血清样本在去除补体活性和外源病毒后的中和能力。该程序通常在56 ℃下灭活病毒30 min后进行。尽管该方案已被广泛接受用于包膜RNA病毒的灭活，但最近的研究表明，需要延长热灭活时间才能完全灭活两种甲病毒，即西方马脑炎病毒和CHIKV。病毒灭活不完全会造成实验室生物安全风险，还可能导致虚假的检测结果。尽管甲病毒的热稳定性在确保实验室人员的安全性以及试验完整性方面很重要，但尚未对甲病毒的热稳定性进行系统研究。在这项研究中，RRV，Barmah森林，和o'nyong-nyong病毒的温度耐受性和热灭活概况进行了测定。在塞姆利基森林血清复合物中观察到热稳定性的变化。因此，建议对甲病毒采用循证热灭活程序。(C)© 2016 Elsevier B.V.版权所有。
Serological diagnosis is a critical component for disease surveillance and is important to address the increase in incidence and disease burden of alphaviruses, such as the chikungunya (CHIKV) and Ross River (RRV) viruses. The gold standard for serological diagnosis is the plaque reduction neutralization test (PRNT), which demonstrates the neutralizing capacity of serum samples after the removal of complement activity and adventitious viruses. This procedure is normally performed following inactivation of the virus at 56 degrees C for 30 min. Although this protocol has been widely accepted for the inactivation of envelope RNA viruses, recent studies have demonstrated that prolonged heat inactivation is required to completely inactivate two alphaviruses, Western equine encephalitis virus and CHIKV. Incomplete inactivation of viruses poses a laboratory biosafety risk and can also lead to spurious test results. Despite its importance in ensuring the safety of laboratory personnel as well as test integrity, systematic investigation on the thermostability of alphaviruses has not been performed. In this study, the temperature tolerance and heat inactivation profiles of RRV, Barmah Forest, and o'nyong-nyong viruses were determined. Variations in thermostability were observed within the Semliki forest serocomplex. Therefore, evidence-based heat inactivation procedures for alphaviruses are recommended. (C) 2016 Elsevier B.V. All rights reserved.