GENETIC-REGULATION OF MEC-3 GENE-EXPRESSION IMPLICATED IN THE SPECIFICATION OF THE MECHANOSENSORY NEURON CELL-TYPES IN CAENORHABDITIS-ELEGANS
GENETIC-REGULATION OF MEC-3 GENE-EXPRESSION IMPLICATED IN THE SPECIFICATION OF THE MECHANOSENSORY NEURON CELL-TYPES IN CAENORHABDITIS-ELEGANS
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DOI：
10.1046/j.1440-169x.1995.t01-4-00010.x
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发表时间：
1995-10-01
影响因子：
2.5
通讯作者：
MITANI, S
中科院分区：
文献类型：
--
作者：
MITANI, S
Mec-3基因是线虫触觉感受器、FLP和PVD神经元分化所必需的，属于LIM同源结构域转录因子。用紫外光照射带有染色体外DNA的不稳定转基因菌株，获得了稳定整合的mec-3-lacZ融合转基因菌株。在改变触摸受体特异性基因表达的突变背景(LIN-4、LIN-14、EGL-44、EGL-46和sem-4基因)中，研究了mec-3-lacZ融合的表达模式。在lin-4突变背景中，9%的动物观察到异位的mec-3-lacZ阳性的AVM/PVM样细胞。相比之下，在Lin-14突变背景下，86%的动物AVM/PVM细胞中的mec-3-lacZ染色消失。在EGL-44和EGL-46突变背景中，表达模式与野生型动物相同。在sem-4突变体背景中，超过一半的动物(54-69%)除了野生型染色模式外，还在尾部有异位染色细胞。提出了这些基因相互作用在机械感觉神经元分化中的作用模式。
The mec-3 gene, a member of the LIM-homeodomain transcription factors, is required for touch receptor, FLP and PVD neurons to differentiate in the nematode Caenorhabditis elegans. Stably integrated transgenic strains with mec-3-lacZ fusion were generated by irradiating UV light to an unstable transgenic strain with the extrachromosomal DNA. Expression patterns of the mec-3-lacZ fusion were examined in mutant backgrounds (lin-4, lin-14, egl-44, egl-46 and sem-4 genes) which alter touch receptor-specific gene expression. In the lin-4 mutant background, ectopic mec-3-lacZ positive AVM/PVM-like cells were observed in 9% of the animals. By contrast, in the lin-14 mutant background, mec-3-lacZ staining in AVM/PVM cells was lost in 86% of the animals. In the egl-44 and egl-46 mutant backgrounds, expression pattern was the same as wild-type animals. In the sem-4 mutant background, more than half of the animals (54-69%) had ectopic staining cells in the tail in addition to the wild-type staining pattern. The modes of action of these genetically interacting genes in the differentiation of mechanosensory neurons are proposed.