Surface appendages, hemagglutination, and adherence to human epithelial cells of Bacteroides intermedius.
Surface appendages, hemagglutination, and adherence to human epithelial cells of Bacteroides intermedius.
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中间拟杆菌的表面附属物、血凝和对人上皮细胞的粘附。
DOI：
10.1111/j.1399-302x.1989.tb00253.x
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发表时间：
1989
影响因子：
--
通讯作者：
Clark,WB
中科院分区：
文献类型：
--
作者：
Leung,KP;Fukushima,H;Sagawa,H;Walker,CB;Clark,WB
在中间拟杆菌口腔菌株上发现了四种形态不同的表面附属物。这些附属物尺寸不同，分别为A、B、C和D，直径分别为1-2 nm、12 nm、8 nm和5 nm。通过电子显微镜检查了 20 个不同的菌株，并选择了 5 个菌株（5、17、27、113 和 25611）来代表所遇到的不同附属物。 A 型附属物是排列在细胞表面的细丝，主要与菌株 5、113 和 ATCC 25611 相关。B 型附属物存在于所有 5 个菌株上，但这些结构很少（即每个生物体 3 个）。 C 型附属物仅与菌株 17 相关。D 型附属物主要存在于菌株 27 上。还测试了这些生物体的血凝活性及其粘附人类口腔上皮细胞的能力。菌株 17 具有 C 型附属物（其他菌株不常见），与 5 种不同种类的红细胞强烈凝集，并强烈粘附在人口腔上皮细胞上。其他菌株具有不同类型的附属物，在血凝活性和粘附特性方面表现出相当大的差异。推测了这些表面附属物和粘附功能之间的相关性。
Four types of morphologically distinct surface appendages were found on oral strains ofBacteroides intermedius.These appendages, designated as A, B, C, and D, were different in size, with diameters of 1–2 nm, 12 nm, 8 nm, and 5 nm, respectively. Twenty different strains were examined by electron microscopy and 5 strains, 5, 17, 27, 113, and 25611, were selected to be representative of the different appendages encountered. Type A appendages were thin filaments per‐itrichously arranged on the cell surface and were associated mainly with Strains 5, 113, and ATCC 25611. Type B appendages were present on all 5 strains but these structures were scarce (i.e., 3 per organism). Type C appendages were associated exclusively with Strain 17. Type D appendages were present mainly on Strain 27. Hemagglulination activity of these organisms and their ability to adhere to human buccal epithelial cells were also tested. Strain 17, which possessed Type C appendage (uncommon to the other strains), agglutinated strongly with 5 different species of erythrocytes and adhered avidly to human buccal epithelial cells. The other strains, possessing different types of appendages, showed considerable variation in hemagglutination activity and adherence properties. Correlation between these surface appendages and adherent functions was speculated.