A comparison of aromatase, 5α‐, and 5β‐ reductase activities in the brain and pituitary of male and female quail (C. c. japonica)
A comparison of aromatase, 5α‐, and 5β‐ reductase activities in the brain and pituitary of male and female quail (C. c. japonica)
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雄性和雌性鹌鹑（C. c. japonica）大脑和垂体中芳香酶、5α-和 5β-还原酶活性的比较
DOI：
10.1002/jez.1402420208
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发表时间：
1987
影响因子：
--
通讯作者：
G. Callard
中科院分区：
文献类型：
--
作者：
B. Schlinger;G. Callard
在包括日本鹌鹑（Coturnix coturnix japonica）在内的许多脊椎动物中，睾酮（T）对神经内分泌靶组织的作用部分是通过转化为雌激素和雄激素代谢产物来介导的。为了评估哪些途径在鹌鹑脑中每个确定的雄激素靶区域中是有利的，以及是否存在可辨别的性别差异，我们开发了一种同时定量芳香酶、5 α-还原酶和5 β-还原酶的测定。此外，我们首次明确鉴定了鹌鹑脑垂体中的芳香化酶，并比较了雄、雌鹌鹑脑垂体中这三种酶的活性。通过将[3 H]雄烯二酮转化为[3 H]雌酮、[3 H]5 α-雄烷二酮和5 β-雄烷二酮来测量组织匀浆中的酶。芳香化酶活性仅限于边缘组织（前下丘脑大于后下丘脑大于隔大于archistriatum含有带核），而上纹状体，小脑，中脑含有核intercollicularis芳香化酶阴性。鹌鹑垂体芳香化雄激素的速率相当于下丘脑前部/视前区（aHPOA）。5 α-和5 β-还原酶存在于所有测试的组织中。芳香化酶显着较高的aHPOA和垂体的男性，而5 α-还原酶显着较高的女性垂体。这些数据表明，一个复杂的雄激素代谢酶控制神经内分泌组织中的活性激素的神经解剖（空间）分布，男性和女性之间的数量差异可能会导致行为的性别差异。
In numerous vertebrate species including Japanese quail (Coturnix coturnix japonica), actions of testosterone (T) on neuroendocrine target tissues are mediated in part by conversion to estrogenic and androgenic metabolites. In order to assess which pathways were favored in each identified androgen target area in quail brain and whether there were discernible sex differences, we developed an assay for simultaneously quantifying aromatase, 5 alpha-, and 5 beta-reductase. In addition, we made the first definitive identification of aromatase in quail pituitary and compared all three enzyme activities in the pituitary of males and females. Enzymes were measured in tissue homogenates by the conversion of [3H]androstenedione to [3H]estrone, [3H]5 alpha-androstanedione, and 5 beta-androstanedione. Aromatase activity was restricted to limbic tissues (anterior hypothalamus greater than posterior hypothalamus greater than septum greater than archistriatum containing nucleus taenia) while hyperstriatum, cerebellum, and midbrain containing nucleus intercollicularis were aromatase-negative. Quail pituitary aromatized androgen at rates equivalent to anterior hypothalamus/pre-optic area (aHPOA). 5 alpha- and 5 beta-reductase were present in all tissues tested. Aromatase was significantly higher in aHPOA and pituitary of males, whereas 5 alpha-reductase was significantly higher in female pituitary. These data suggest that a complex of androgen-metabolizing enzymes controls the neuroanatomic (spatial) distribution of active hormone in neuroendocrine tissues and that quantitative differences between males and females may account for sex differences in behavior.
围产期发育过程中雪貂大脑中雄激素芳香化和 5 α 还原：性和睾酮控制的影响。
影响因子：
4.8
作者：
Tobet,SA;Shim,JH;Osiecki,ST;Baum,MJ;Canick,JA
通讯作者：
Canick,JA
啮齿动物垂体细胞株中芳香酶活性的鉴定。
影响因子：
4.8
作者：
Callard,GV;Petro,Z;TashjianJr,AH
通讯作者：
TashjianJr,AH