Nonrandom distribution of metaphase AgNOR staining patterns on human acrocentric chromosomes
Nonrandom distribution of metaphase AgNOR staining patterns on human acrocentric chromosomes
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DOI：
10.1177/002215540004800102
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发表时间：
2000-01-01
影响因子：
3.2
通讯作者：
Usson, Y
中科院分区：
文献类型：
--
作者：
Héliot, L;Mongelard, F;Usson, Y
中期核仁组织区 (NOR) 包含与上游结合因子 (UBF) 和 RNA 聚合酶 I (RPI) 等蛋白质相关的核糖体基因。这些基因聚集在人类近端着丝粒染色体的 10 个基因座（13、14、15、21 和 22）中。一些 NOR 相关蛋白（称为 AgNOR 蛋白）可以被银特异性染色。在这项研究中，我们利用数字成像、图像恢复技术和因子对应分析 (FCA) 方面的技术进步来研究人类淋巴细胞中期染色体的不同 AgNOR 染色模式。可以区分三种主要模式：成对（47%）、光滑状（28%）和未染色（18%）结构。通过研究不同染色体上每种模式的出现频率，可以定义两个群体。 13、14和21号染色体主要携带成对或棒状AgNOR结构，而15和22号染色体主要携带成对AgNOR结构或保持未染色。我们认为不同的 AgNOR 形状既反映了每条染色体携带的核糖体基因的数量，也反映了每个 NOR 簇中活性核糖体基因的差异招募。这是第一项显示 AgNOR 形状在近端着丝粒染色体中非随机分布的研究。
The metaphase nucleolar organizer regions (NORs) contain ribosomal genes associated with proteins such as upstream binding factor (UBF) and RNA polymerase I (RPI). These genes are clustered in 10 loci of the human acrocentric chromosomes (13, 14, 15, 21, and 22). Some NOR-associated proteins, termed AgNOR proteins, can be specifically stained by silver. In this study we took advantage of technical advances in digital imaging, image restoration techniques, and factorial correspondence analysis (FCA) to study the different AgNOR staining patterns of metaphase chromosomes in human lymphocytes. Three predominant patterns could be distinguished: pair (47%), slick-like (28%), and unstained (18%) structures. By studying the frequency of occurrence of each pattern on different chromosomes, two groups could be defined. Chromosomes 13, 14, and 21 carried predominantly pair or stick-like AgNOR structures, whereas chromosomes 15 and 22 mainly carried pair AgNOR structures or remained unstained. We suggest that the different AgNOR shapes reflect both the number of ribosomal genes carried by each chromosome and the differential recruitment of active ribosomal genes in each NOR cluster. This is the first study showing a nonrandom distribution of AgNOR shape among acrocentric chromosomes.