Overexpression of wheat NF-YA10 gene regulates the salinity stress response in Arabidopsis thaliana
Overexpression of wheat NF-YA10 gene regulates the salinity stress response in Arabidopsis thaliana
复制标题
小麦NF-YA10基因过表达调控拟南芥盐胁迫反应
DOI：
10.1016/j.plaphy.2014.11.011
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发表时间：
2015-01-01
影响因子：
6.5
通讯作者：
Wang, Mei
中科院分区：
文献类型：
--
作者：
Ma, Xiaoyan;Zhu, Xinlei;Wang, Mei
核因子Y（NF-Y）转录因子由三个不同的亚基（NF-YA、-YB和-YC）相互作用形成。它靶向CCAAT盒，这是真核启动子中常见的顺式元件。在此，面包小麦基因TaNF-YA 10 -1已从耐盐栽培品种SR 3中分离。重组TaNF-YA 10 -1在大肠杆菌中异源产生，纯化的蛋白在体外成功地结合到CCAAT基序。TaNF-YA 10 -1在盐胁迫和脱落酸（阿坝）胁迫下表达下调。从种子萌发、子叶变绿和根的相对生长来看，TaNF-YA 10 -1在拟南芥中的组成型表达显著提高了植物对盐的敏感性，抑制了其对ABA的敏感性。在TaNF-YA 10 -1过表达转基因植株中，胁迫相关基因AtRAB 18、AtRD 29 B、AtABI 5、AtCBF 1和AtCBF 3的转录水平下调。这些数据为TaNFYA 10 -1参与盐胁迫条件下生长的调节提供了支持性证据。(C)2014年Elsevier Masson SAS。All rights reserved.
The nuclear factor Y (NF-Y) transcription factor is formed by the interaction of three distinct subunits (NF-YA, -YB and -YC). It targets the CCAAT box, a common cis-element in eukaryotic promoters. Here, the bread wheat gene TaNF-YA10-1 has been isolated from the salinity tolerant cultivar SR3. Recombinant TaNF-YA10-1 was heterologously produced in Escherichia coli, and the purified protein successfully bound to the CCAAT motif in vitro. TaNF-YA10-1 was down-regulated by the imposition of salinity and abscisic acid (ABA). The constitutive expression of TaNF-YA10-1 in Arabidopsis thaliana significantly increased the plant's sensitivity to salinity and repressed its sensitivity to ABA as judged from the seed germination, cotyledon greening and the relative root growth. The transcription of stress-related genes AtRAB18, AtRD29B, AtABI5, AtCBF1 and AtCBF3 was downregulated in TaNF-YA10-1 overexpression transgenic plants. The data provide supportive evidence that TaNFYA10-1 is involved in the regulation of growth under salinity stress conditions. (C) 2014 Elsevier Masson SAS. All rights reserved.