Axonal growth from the habenular nucleus along the neuromere boundary region of the diencephalon is regulated by semaphorin 3F and netrin-1
Axonal growth from the habenular nucleus along the neuromere boundary region of the diencephalon is regulated by semaphorin 3F and netrin-1
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DOI：
10.1006/mcne.2000.0870
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发表时间：
2000-09-01
影响因子：
3.5
通讯作者：
Takahashi, H
中科院分区：
文献类型：
--
作者：
Funato, H;Saito-Nakazato, Y;Takahashi, H
在神经发育中，主要的神经束通常沿着神经节边界区域形成，尽管这种形成的分子机制仍有待阐明。在间脑中，来自缰核的轴突沿着p1和p2之间的神经节边界区域延伸。在胚胎13-15天，在3类semaphorins的成员中，只有semaphorin 3F（Sema 3F）在间脑中表达。Sema 3F，这是强烈表达的喙p1，排斥轴突从缰外植体。虽然P2外植体没有施加从缰外植体在一定距离的轴突上的排斥作用，缰轴突没有生长到P2外植体。从表达netrin-1的间脑尾部的腹侧区域的外植体吸引来自缰外植体的轴突。DCC的抗体可阻断这种引诱作用。这些结果表明，从缰核的轴突的生长沿着的神经节边界区域可能是由Sema 3F从吻侧P1，和netrin-1从腹侧区域的尾部间脑。
In neural development, major tracts are often formed along the neuromere boundary regions, although the molecular mechanism underlying this formation remains to be clarified. In the diencephalon, axons from the habenular nucleus extend along the neuromere boundary region between p1 and p2. At embryonic days 13-15, among members of class 3 semaphorins, only semaphorin 3F (Sema3F) was expressed in the diencephalon. Sema3F, which was strongly expressed in the rostral p1, repulsed axons from habenular explants. While p2 explants did not exert a repulsive effect on axons from habenular explants at a distance, habenular axons did not grow into p2 explant. Explants from the ventral region of the caudal diencephalon where netrin-1 is expressed attracted the axons from habenular explants. The attractive effect was blocked by an antibody for DCC. These results suggest that the growth of axons from the habenular nucleus along the neuromere boundary region may be regulated by Sema3F from the rostral p1, and netrin-1 from the ventral region of the caudal diencephalon.