A microfluidic chip for heterogeneous immunoassay using electrokinetical control
A microfluidic chip for heterogeneous immunoassay using electrokinetical control
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DOI：
10.1007/s10404-005-0040-0
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发表时间：
2005-10-01
影响因子：
2.8
通讯作者：
Li, DQ
中科院分区：
文献类型：
--
作者：
Hu, GQ;Gao, YL;Li, DQ
本文介绍了在聚二甲基硅氧烷（PDMS）微流控芯片上开发的一种新型、自动化、电动控制的异质免疫分析方法。已经开发了一种数值方法来模拟复杂微通道网络中试剂和洗涤溶液的电动驱动、时间依赖的输送过程。基于数值模拟确定的参数，通过依次改变外加电场，实现了对幽门螺杆菌的全自动化片上检测实验。通过使用这种微通道芯片，缩短了分析时间，减少了试剂消耗，同时检测限与传统分析相当。实验结果与数值预测吻合较好，证明了利用CFD辅助微流控免疫分析实验研究的有效性。
This article presents the development of a novel, automated, electrokinetically controlled heterogeneous immunoassay on a poly(dimethylsiloxane) (PDMS) microfluidic chip. A numerical method has been developed to simulate the electrokinetically driven, time-dependent delivery processes of reagents and washing solutions within the complex microchannel network. Based on the parameters determined from the numerical simulations, fully automated on-chip experiments to detect Helicobacter pylori were accomplished by sequentially changing the applied electric fields. Shortened assay time and much less reagent consumptions are achieved by using this microchannel chip while the detection limit is comparable to the conventional assay. There is a good agreement between the experimental result and numerical prediction, demonstrating the effectiveness of using CFD to assist the experimental studies of microfluidic immunoassay.