The luteinizing hormone-releasing hormone (LHRH) systems in the rat brain.
The luteinizing hormone-releasing hormone (LHRH) systems in the rat brain.
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DOI：
10.1159/000123419
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发表时间：
1982-07
影响因子：
4.1
通讯作者：
J. Witkin;C. Paden;A. Silverman
中科院分区：
文献类型：
--
作者：
J. Witkin;C. Paden;A. Silverman
在未包埋的厚切片上使用免疫细胞化学方法观察大鼠中枢神经系统（CNS）中含有lhrh免疫反应物质的神经元及其过程。在用秋水仙碱（75微克，脑室内）预处理的动物中，可以观察到远至嗅球前部和远至下丘脑基底交叉后区域后部的细胞体。本研究对大鼠进行了一些新的观察，包括在副嗅球和其他嗅觉相关结构中，以及在海马前部和灰脑中发现LHRH神经元。与其他实验室的研究一样，我们在脑室周围内侧视前区、布洛卡核和间隔核的对角线带观察到许多LHRH细胞，而在下丘脑前部区和视上连接区观察到较少的阳性细胞。来自所有这些细胞的LHRH纤维广泛分布在中枢神经系统中。除了密集的正中隆起神经支配外，阳性纤维还可支配其他心室周围器官，它们靠近心室系统室管膜壁或与脑动脉、脑膜和蛛网膜下腔密切相关。LHRH纤维也可以支配中枢神经系统的几个区域的神经元。在大鼠中发现了一种新的LHRH纤维投射，起源于胼胝体上神经元，并通过扣带皮层和新皮层。讨论了各LHRH细胞群传出信号的可能分布。
Immunocytochemical procedures on thick, unembedded sections were used to visualize the neurons and their processes that contain LHRH-immunoreactive material in the rat central nervous system (CNS). In animals pretreated with colchicine (75 micrograms, intraventricularly), cell bodies could be observed as far anterior as the olfactory bulb and posterior to the retrochiasmatic area of the basal hypothalamus. Several new observations for the rat were made in this study, including LHRH neurons in the accessory olfactory bulb and other olfactory-related structures, and in the anterior hippocampus and the induseum griseum. As in studies from other laboratories, we observed many LHRH cells in the periventricular medial preoptic area, diagonal band of Broca and septal nuclei, and fewer positive cells in the anterior hypothalamic area and the region of the supraoptic commissure. The LHRH fibers from all of these cells are widely dispersed in the CNS. In addition to the dense innervation of the median eminence, positive fibers are found innervating other circumventricular organs, coursing close to the ependymal wall of the ventricular system or in close association with cerebral arteries and areas of the pia mater and subarachnoid space. LHRH fibers may also innervate neurons in several regions of the CNS. A novel projection of LHRH fibers for the rat was found originating from supracallosal neurons and coursing through both cingulate and neocortex. The possible distribution of efferents from each LHRH cell group is discussed.