EXPRESSION OF MESSENGER RIBONUCLEIC-ACIDS ENCODING A PARATHYROID HORMONE-LIKE PEPTIDE IN NORMAL HUMAN AND ANIMAL-TISSUES WITH ABNORMAL EXPRESSION IN HUMAN PARATHYROID ADENOMAS
EXPRESSION OF MESSENGER RIBONUCLEIC-ACIDS ENCODING A PARATHYROID HORMONE-LIKE PEPTIDE IN NORMAL HUMAN AND ANIMAL-TISSUES WITH ABNORMAL EXPRESSION IN HUMAN PARATHYROID ADENOMAS
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DOI：
10.1210/mend-2-12-1230
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发表时间：
1988-12-01
影响因子：
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通讯作者：
BROADUS, AE
中科院分区：
文献类型：
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作者：
IKEDA, K;WEIR, EC;BROADUS, AE
最近从与恶性肿瘤体液性高钙血症综合征相关的人类肿瘤中纯化并克隆了一种新的PTH样肽。我们用北方分析法检测了编码该肽的mRNA在正常组织中的表达。从人角质形成细胞、甲状腺、骨髓和成纤维细胞、牛下丘脑、垂体、甲状旁腺、肾上腺皮质和肾上腺髓质以及大鼠脑、胃粘膜和胎儿肝脏（而非成人肝脏）制备的poly（A）+ RNA中鉴定出一种或多种低丰度杂交转录物。在从许多已建立的细胞系制备的poly（A）+ RNA中也鉴定出一种或多种杂交转录物，所述细胞系包括大鼠垂体（GH4）、大鼠嗜铬细胞瘤（PC 12）、人骨肉瘤（TE-85）和人髓样癌（TT）细胞。对来自异常人甲状旁腺组织的mRNA的北方分析揭示了PTH样肽的转录物的过表达，该肽似乎对腺瘤性或自主性腺体具有特异性。这些研究结果表明，PTH样肽在许多内分泌和非内分泌组织中表达，它在发育过程中至少在一种组织（胎肝）中表达，并且其表达的调节在人甲状旁腺腺瘤中是异常的。
A novel PTH-like peptide has recently been purified and cloned from human tumors associated with the syndrome of humoral hypercalcemia of malignancy. We surveyed the expession of mRNAs encoding this peptide in normal tissues by Northern analysis. One or more low abundance hybridizing transcripts was identified in poly(A)+ RNA prepared from human keratinocytes, thyroid, bone marrow, and fibroblasts, from bovine hypothalamus, pituitary, parathyroid, adrenal cortex, and adrenal medulla, and from rat brain, stomach mucosa, and fetal but not adult liver. One or more hybridizing transcripts was also identified in poly(A)+ RNA prepared from a number of established lines, including rat pituitary (GH4), rat pheochromocytoma (PC 12), human osteosarcoma (TE-85), and human medullary carcinoma (TT) cells. Northern analysis of mRNAs from abnormal human parathyroid tissue revealed an overexpression of transcripts for the PTH-like peptide which appeared to be specific for adenomatous or autonomous glands. These findings suggest that the PTH-like peptide is expressed in a number of endocrine and nonendocrine tissues, that it is developmentally expressed in at least one tissue (fetal liver), and that the regulation of its expression is abnormal in human parathyroid adenomas.