Hepatitis E viral infection causes testicular damage in mice
Hepatitis E viral infection causes testicular damage in mice
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戊型肝炎病毒感染导致小鼠睾丸损伤
DOI：
10.1016/j.virol.2019.12.009
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发表时间：
2020-02-01
期刊：
影响因子：
3.7
通讯作者：
Huang, Fen
中科院分区：
文献类型：
--
作者：
Situ, Jianwen;Wang, Wenjing;Huang, Fen
戊型肝炎病毒（HEV）是戊型肝炎的主要病原体，具有多个肝外复制位点。不育男性精液中HEV RNA的存在表明HEV在男性生殖道中复制。然而，这一机制在很大程度上仍然难以捉摸。采用BALB/c动物模型，观察戊型肝炎病毒（HEV）感染对睾丸的损伤作用。接种后7 ~ 28 d，粪便、血液和肝脏中检出HEV RNA，7 ~ 70 d，男性生殖道中检出HEV RNA。在睾丸、附睾和精囊中均观察到HEV抗原阳性信号。精子质量下降，血睾屏障（BTB）破坏，精原细胞数量急剧减少，提示HEV感染可引起睾丸损伤。睾丸中几乎未发现抗病毒免疫反应。结果表明，戊型肝炎病毒在男性生殖道复制，造成睾丸损伤，从而导致生育缺陷。
Hepatitis E virus (HEV) is the main pathogen of hepatitis E infections with multiple extrahepatic replication sites. The presence of HEV RNA in the semen of infertile males suggests HEV replicates in the male genital tract. However, the mechanism is largely remained elusive. A BALB/c-based animal model was used to evaluate the effects of HEV infection on the testicular damage. HEV RNA was detected in feces, blood and livers from 7 to 28 days post-inoculation (dpi), while was positive in male genital tract from 7 to 70 dpi. Positive signals of HEV antigens were observed in testes, epididymides and seminal vesicles (SVs). Impaired sperm quality, destroyed the blood-testis barrier (BTB) and drastically decreased spermatogonia suggested that HEV infection causes testicular damage. Antiviral immune response was barely found in the testes. Results demonstrated that HEV replicates in male genital tract, causes testicular damage, and consequently results in flawed fertility.