Probing the Chemo-Mechanical Effects of an Anti-Cancer Drug Emodin on Breast Cancer Cells
Probing the Chemo-Mechanical Effects of an Anti-Cancer Drug Emodin on Breast Cancer Cells
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DOI：
10.1007/s12195-011-0163-1
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发表时间：
2011-09-01
影响因子：
2.8
通讯作者：
Lim, Chwee Teck
中科院分区：
文献类型：
--
作者：
Wuang, Shy Chyi;Ladoux, Benoit;Lim, Chwee Teck
抗癌药物大黄素可引起人类癌细胞的显著机械生物学变化，这是由于该药物的抗细胞粘附、肌动蛋白破坏和促凋亡作用的相互作用。在这里，我们使用微柱来监测乳腺癌细胞对这种药物刺激的反应引起的牵引力的变化。我们的研究结果表明，细胞牵引力的变化反映了细胞骨架完整性和细胞行为的变化。发现细胞牵引力在细胞变圆过程中增加，在细胞凋亡和细胞骨架破坏发生过程中减少。这些结果可以提供有用的见解所产生的细胞-基质相互作用的化学-力学关系，并可能有助于药物疗效的定量评估。
The anti-cancer drug, emodin, can cause significant mechanobiological changes in human cancer cells, due to the interplay of anti-cell adhesion, actin-disrupting and apoptosis-causing effects of the drug. Here, we used micropillars to monitor the changes in traction forces induced by breast cancer cells in response to this drug stimulus. Our results showed that the variations in the cell traction forces reflected changes in the cytoskeleton integrity and cell behavior. Cell traction forces were found to increase during cell rounding and decrease during the onset of apoptosis and cytoskeleton disruption. These results can provide useful insights into the chemo-mechanical relationships arising from the cell-matrix interactions and can potentially help in the quantitative assessment of drug efficacy.