Effect of water temperature during ultrasonic denture cleaning
Effect of water temperature during ultrasonic denture cleaning
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DOI：
10.2334/josnusd.17-0387
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发表时间：
2019-03-01
影响因子：
1.9
通讯作者：
Ichikawa, Tetsuo
中科院分区：
文献类型：
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作者：
Iwawaki, Yuki;Matsuda, Takashi;Ichikawa, Tetsuo
义齿菌斑是由各种微生物与唾液聚集而成的生物膜。开发和研究了各种义齿清洗剂和清洁器具。然而，清洁义齿的最佳水温是未知的。因此，本研究探讨了水温对超声义齿清洗的影响。体外，在不同温度下用水超声清洗5 min后，将带有人工白色念珠菌生物膜的树脂片压在念珠菌GE培养基上。培养基随后在37℃下培养24小时。使用ImageJ软件（美国国立卫生研究院，Bethesda， MD， USA）观察形成的菌落，并对菌落面积进行量化。原位测定不同温度水超声清洗5 min前后上颌义齿组织表面细菌数量及洁净度。计算不同温度下细菌数量和清洁度的变化。温水清洗后菌落在体外的面积占比和原位还原率明显低于冷水清洗后的菌落占比。因此，用温水进行超声义齿清洗更为有效。
Denture plaque is a biofilm composed of various microorganisms aggregated with saliva. Various denture cleansers and cleaning apparatuses have been developed and studied. However, the optimum water temperature for denture cleaning is unknown. Therefore, the present study investigated the effects of water temperature during ultrasonic denture cleaning. In vitro, resin disks with artificial Candida albicans biofilm were pressed onto Candida GE media after ultrasonic cleaning with water at different temperatures for 5 min. The media were subsequently cultured at 37 degrees C for 24 h. The colonies formed were observed and colony areas were quantified using ImageJ software (US National Institutes of Health, Bethesda, MD, USA). In situ, the bacterial count and degree of cleanliness on the tissue surface of maxillary dentures were measured before and after ultrasonic cleaning with water at different temperatures for 5 min. Changes in bacterial counts and cleanliness were calculated for each temperature. The ratio of the area occupied by bacterial colonies in vitro and reduction rates in situ after cleaning with warm water were markedly less than those observed after cleaning with cold water. Therefore, ultrasonic denture cleaning with warm water is more effective.