Coexpression of granulocyte colony stimulating factor and its receptor in primary ovarian carcinomas
Coexpression of granulocyte colony stimulating factor and its receptor in primary ovarian carcinomas
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DOI：
10.1016/s0304-3835(00)00623-6
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发表时间：
2001-01-10
期刊：
影响因子：
9.7
通讯作者：
Quesenberry, PJ
中科院分区：
文献类型：
--
作者：
Savarese, TM;Mitchell, K;Quesenberry, PJ
采用免疫组织化学方法检测粒细胞集落刺激因子（G-CSF）及其受体（G-CSFR）在原发性卵巢癌中的表达。G-CSFR的表达中观察到的恶性细胞的46个原发癌检查; G-CSF的共同表达的恶性上皮细胞和间质的标本的56.5%。因此，大多数卵巢癌同时具有潜在的自分泌和旁分泌G-CSF轴。在37%的样本中，G-CSF仅在基质细胞内表达，这表明只有潜在的旁分泌系统存在。在一项初步的回顾性评价中，肿瘤仅表达明显的旁分泌环的患者的生存期明显低于肿瘤表达潜在的自分泌和旁分泌G-CSF调节环的患者（分别为14.5和42.5个月）。使用表达G-CSFR的OVCAR-3卵巢癌细胞系对G-CSF的潜在功能进行研究。rhG-CSF单药不能刺激[H-3]-胸苷掺入细胞，但能增强表皮生长因子（EGF）的促有丝分裂作用，且呈剂量依赖性。因此，潜在的自分泌和/或旁分泌循环涉及G-CSF及其受体发生在90%以上的原发性卵巢癌，并可能发挥调节生长因子的作用。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Immunohistochemistry was used to determine the expression of granulocyte colony-stimulating factor (G-CSF) and its receptor (G-CSFR) in primary ovarian carcinomas. The expression of G-CSFR was observed in the malignant cells of each of the 46 primary carcinomas examined; G-CSF was coexpressed in both the malignant epithelial cells and the stroma of 56.5% of the specimens. Thus the majority of ovarian carcinomas harbor both potential autocrine and paracrine G-CSF axes. In 37% of the samples, G-CSF was expressed only within stromal cells, suggesting that only a potential paracrine system is in place. In a preliminary, retrospective, evaluation, the survival of patients whose tumors expressed only the apparent paracrine loop was significantly worse than patients whose tumors expressed both potential autocrine and paracrine G-CSF-based regulatory loops (14.5 vs. 42.5 months, respectively). Studies on the potential function of G-CSF were performed using the G-CSFR-expressing OVCAR-3 ovarian carcinoma line. As a single agent, rhG-CSF failed to stimulate [H-3]-thymidine incorporation in these cells, but enhanced the mitogenic action of epidermal growth factor (EGF) in a dose-dependent manner. Thus, potential autocrine and/or paracrine loops involving G-CSF and its receptor occur in over 90% of primary ovarian carcinomas, and may act to modulate the action of growth factors. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.