B-LYMPHOPOIESIS ON STROMAL CELL CLONE - STROMAL CELL CLONES ACTING ON DIFFERENT STAGES OF B-CELL DIFFERENTIATION
B-LYMPHOPOIESIS ON STROMAL CELL CLONE - STROMAL CELL CLONES ACTING ON DIFFERENT STAGES OF B-CELL DIFFERENTIATION
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DOI：
10.1002/eji.1830181117
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发表时间：
1988-11-01
影响因子：
5.4
通讯作者：
KODAMA, H
中科院分区：
文献类型：
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作者：
NISHIKAWA, SI;OGAWA, M;KODAMA, H
比较了两个基质细胞克隆 MC3T3-G2/PA6 (PA6) 和 ST2 的 B 淋巴细胞生成支持活性。当在Whitlock-Witte型条件下在这些克隆中培养正常骨髓细胞时，仅在具有ST2层的培养物中产生成熟B细胞。然而，保留在 PA6 层上的细胞含有当转移到 ST2 层时产生成熟 B 细胞的前体细胞。因此，PA6 是一种基质细胞克隆，能够支持早期 B 祖细胞，但不能支持进一步成熟为前 B 细胞。 PA6 层上维持的 B 祖细胞的免疫球蛋白重链基因构型在转移到 ST2 层后发生了多样化。这表明它们实际上是最早的祖先。 PA6 和 ST2 之间基质细胞活性的显着差异也可以通过基质细胞依赖性前 B 细胞系来区分。在测试的四种 ST2 依赖性前 B 细胞系中，两种仅在 ST2 层上生长，能够支持 B 淋巴细胞生成，而其他则同时在 ST2 和 PA6 层上生长。这些结果强烈表明，骨髓内 B 细胞发育过程是由作用于 B 细胞分化不同阶段的不止一种信号控制的。
B lymphopoiesis supporting activities of two stromal cell clones, MC3T3-G2/PA6 (PA6) and ST2, were compared. When normal bone marrow cells were cultured in these clones under Whitlock-Witte-type condition, mature B cells were generated only in the culture with the ST2 layer. The cells maintained on the PA6 layer, however, contained the precursor cells giving rise to mature B cells when transferred to the ST2 layer. Thus, PA6 is a stromal cell clone capable of supporting the early B progenitos but cannot support a further maturation step into pre-B cells. The immunoglobulin heavy chain gene configuration of B progenitors maintained on the PA6 layer diversified after their transfer onto ST2 layer. This suggests that they are actually the earliest progenitors. This marked difference in the stromal cell activities between PA6 and ST2 could also be distinguished by stromal cell-dependent pre-B cell lines. Among four ST2-dependent pre-B cells lines tested, two grew only on the ST2 layer, which is capable of supporting B lymphopoiesis, while the others grew both on the ST2 and PA6 layers. These results strongly suggest that the process of intramarrow B cell development is controlled by more than one signal acting on different stages of B cell differentiation.