A gene chimaera of SV40 and mouse β-globin is transcribed and properly spliced
A gene chimaera of SV40 and mouse β-globin is transcribed and properly spliced
复制标题
SV40 和小鼠 β-珠蛋白的基因嵌合体被转录并正确剪接
作者：
G. Chu;P. Sharp
A gene chimaera of the first exon from simian virus 40 (SV40) sequences coding for T antigen placed upstream from the third exon from mouse β-globin, and separated by the resultant new chimaeric intron, was cloned into a bacterial plasmid. When transfected into monkey cells, the gene chimaera was transcribed, polyadenylated and spliced using the donor splice site from SV40 and the acceptor splice site from mouse β-globin. This result suggests that a donor site from one gene can be spliced to an acceptor site from another gene.
