Oxygen diffusivity in alginate/chitosan microcapsules
Oxygen diffusivity in alginate/chitosan microcapsules
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DOI：
10.1002/jctb.3845
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发表时间：
2013-03
影响因子：
3.4
通讯作者：
Wei Zhao;Wei Zhao;Ying Zhang;Yang Liu;M. Tan;Weiting Yu;Hongguo Xie;Ying Ma;Ying Ma;Guangwei Sun;Guojun Lv;Zhao Shan;Xiao-jun Ma
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文献类型：
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作者：
Wei Zhao;Wei Zhao;Ying Zhang;Yang Liu;M. Tan;Weiting Yu;Hongguo Xie;Ying Ma;Ying Ma;Guangwei Sun;Guojun Lv;Zhao Shan;Xiao-jun Ma
背景：氧的扩散特性影响细胞在多电解质复合膜微胶囊中的增殖和代谢。计算了不同制备条件下海藻酸盐/壳聚糖（AC）微胶囊中氧的有效扩散系数（De），并建立了氧扩散对微胶囊中细胞负载的影响的数学模型。结果：AC微胶囊中的氧De与藻酸盐溶液浓度、藻酸盐特性粘度及不同聚电解质复合物膜无关。当微胶囊直径从1800 μ m减小到45 μ m时，De从2.1 +/- 0.3 × 109减小到0.17 +/- 0.01 × 109 m2 s1，微胶囊密度从1.013 +/- 0.000 g mL1增大到1.034 +/- 0.003 g mL1。数学模型结果表明，在直径为450 μ m和1800 μ m的微胶囊中，CHO细胞的临界负荷分别为1.8 × 108和1.1 × 108细胞mL1。结论：海藻酸钙微胶囊与AC微胶囊氧De含量无显著差异。De随直径的减小主要是由于表面密度和致密程度的增加。模型结果表明，坏死风险随直径增大而增大。微囊直径越小，在细胞培养中越有优势。(c) 2012年化学工业学会
BACKGROUND: Oxygen diffusion properties affect the proliferation and metabolism of cells cultured in microcapsules with a polyelectrolyte complex membrane. The effective diffusion coefficient (De) of oxygen in alginate/chitosan (AC) microcapsules under different preparation conditions was calculated, and a mathematic model was developed to investigate the effect of oxygen diffusion on cell loading in the microcapsules. RESULTS: Oxygen De in AC microcapsules was independent of alginate solution concentration, intrinsic viscosity of alginate and different polyelectrolyte complex membranes. De decreased from 2.1 +/- 0.3 x 109 to 0.17 +/- 0.01 x 109 m2 s1 as microcapsule diameter decreased from 1800 to 45 0 mu m. Microcapsule density was increased from 1.013 +/- 0.000 to 1.034 +/- 0.003 g mL1 as diameter decreased from 1775 to 430 mu m. The mathematic model results showed that critical CHO cell loadings were 1.8 x 108 or 1.1 x 108 cells mL1 in microcapsules with 450 or 1800 mu m diameter, respectively. CONCLUSIONS: No significant difference was found of oxygen De between calcium alginate beads and AC microcapsules. The decrease of De with diameter was attributed to the increasing density and compact degree on the surface. The model results indicated that risk on necrosis rose with the increasing diameter. Microcapsules with smaller diameters may have more advantages on cell culture. (c) 2012 Society of Chemical Industry