Plant growth promoting endophytic fungi Asprgillus fumigatus TS1 and Fusarium proliferatum BRL1 produce gibberellins and regulates plant endogenous hormones
Plant growth promoting endophytic fungi Asprgillus fumigatus TS1 and Fusarium proliferatum BRL1 produce gibberellins and regulates plant endogenous hormones
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DOI：
10.1007/s13199-018-0545-4
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发表时间：
2018-10-01
期刊：
影响因子：
2.5
通讯作者：
Hussain, Anwar
中科院分区：
文献类型：
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作者：
Bilal, Lubna;Asaf, Sajjad;Hussain, Anwar
内生菌可以作为植物生长促进剂，因为它们分泌大量的植物激素来支持寄主植物。考虑到内生菌的促生长活性，从草叶草（Oxalis corniculata）的根中分离到烟曲霉（Asprgillus fumigatus） TS1和增生镰刀菌（Fusarium proliferatum） BRL1两种内生真菌。对分离株进行了初步的生长促进活性筛选，包括铁载体活性、磷酸盐增溶性、吲哚乙酸和赤霉素的分泌。此外，还测定了这些分离物对水稻突变体Waito-C的生长促进能力。与TS1和BRL1相关的植株具有更高的叶绿素含量、根冠长度和生物量。通过气相色谱/质谱分析（GC/MS），在内生菌培养滤液中发现了多种类型的GAs和IAA，从而促进了内生菌的生长。GC/MS分析显示，TS1和BRL1培养滤液中赤霉素的浓度（ng/ml）不同，分别为GA(1)（0.091 +/- 0.009、0.392 +/- 0.007）、GA(3)（0.324 +/- 0.077、0.089 +/- 0.0007）和GA(7)（0.023 +/- 0.002、0.492 +/- 0.005）。此外，与对照相比，TS1和BRL1相关植株的内源GA(1)显著上调（12.443 +/- 0.454和15.434 +/- 0.245）。此外，半定量RT-PCR证实了GA通路基因（P50-1、P450-3、P450-4、ggs2和des）的存在或参与。结果表明，本研究分离到的内生菌能够合成具有生物活性的化合物，对促进植物生长具有重要作用。
Endophytes can serve as plant growth promoters as they secret a vast array of phytohormones to support host plants. Keeping the growth promoting activity of the endophytes in view, two endophytic fungi, Asprgillus fumigatus TS1 and Fusarium proliferatum BRL1 have been isolated from the roots of Oxalis corniculata. The isolates have been screened initially for growth promoting activities, including siderophores activity, phosphate solubilization, and secreation of indole acetic acid and gibberellins. Further, the isolates have assayed for the ability to promote the growth of mutant rice Waito-C. The plants associated with TS1 and BRL1 have shown higher chlorophyll content, root-shoot length, and biomass production. The growth promoting activity of the endophytes can be attributed to the various types of GAs and IAA that have been observed in the culture filtrates of the endophytes by the Gas chromatography/mass spectrometry (GC/MS). The GC/MS analysis revealed the presence of different gibberellins concentrations (ng/ml) in TS1 and BRL1 culture filtrate, i.e. GA(1) (0.091 +/- 0.009, 0.392 +/- 0.007), GA(3) (0.324 +/- 0.077, 0.089 +/- 0.0007) and GA(7) (0.023 +/- 0.002, 0.492 +/- 0.005), respectively. Besides, a significant up regulation of plant endogenous GA(1) (12.443 +/- 0.454 and 15.434 +/- 0.245) has been obsereved in TS1 and BRL1 associated plants compared to the control. Moreover, semi quantitative RT-PCR has confirmed the presence/invovment of GA pathways genes (P50-1, P450-3, P450-4, ggs2, and des). The results conclude that the endophytes isolated in this study can ably synthesize bioactive compounds, which play an important role in plant growth promotion.