Three-dimensional analysis of mineralizing turkey leg tendon: matrix vesicle-collagen relationships.
Three-dimensional analysis of mineralizing turkey leg tendon: matrix vesicle-collagen relationships.
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矿化火鸡腿腱的三维分析：基质囊泡-胶原蛋白关系。
DOI：
10.1016/s0945-053x(05)80003-4
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Arsenault,AL
中科院分区：
文献类型：
--
作者：
Kohler,DM;Crenshaw,MA;Arsenault,AL
通过对火鸡腿腱的横截面和连续纵向薄切片进行电子显微镜观察，以及对纵向厚切片进行电子断层扫描，研究了基质囊泡和I型胶原原纤维之间矿物沉积的时空关系。连续切片和电子断层摄影允许三维空间关系的分析，克服了缺失深度信息和相邻结构的过度投影的问题，这些问题存在于孤立部分的二维投影中。这些技术表明，虽然发现基质囊泡内的矿物质沉积远离钙化胶原纤维，但不会发生相反的关系;所有胶原相关的矿物质最终都可以与载有矿物质的囊泡联系起来。这些结果表明，钙化的时间顺序开始于基质囊泡和蔓延到相邻的胶原纤维。
The spatial and temporal relationships of mineral deposition between matrix vesicles and type I collagen fibrils have been studied in the turkey leg tendon by electron microscopy of cross sections and serial longitudinal thin sections and by electron tomography of longitudinal thick sections. Serial sectioning and electron tomography allow three-dimensional analysis of spatial relationships, overcoming the problems of missing depth information and over-projection of adjacent structures which exist for two-dimensional projections of isolated sections. These techniques reveal that while mineral deposits within matrix vesicles are found remote from calcifying collagen fibrils, the reverse relationship does not occur; all collagen-associated mineral can ultimately be linked to mineral-laden vesicles. These results suggest a temporal sequence of calcification beginning in matrix vesicles and spreading to adjacent collagen fibrils.