INVIVO UPTAKE OF CHYLOMICRON (H-3)-LABELED RETINYL ESTER BY RAT-LIVER - EVIDENCE FOR RETINOL TRANSFER FROM PARENCHYMAL TO NON-PARENCHYMAL CELLS
INVIVO UPTAKE OF CHYLOMICRON (H-3)-LABELED RETINYL ESTER BY RAT-LIVER - EVIDENCE FOR RETINOL TRANSFER FROM PARENCHYMAL TO NON-PARENCHYMAL CELLS
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DOI：
10.1073/pnas.79.23.7326
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
NORUM, KR
中科院分区：
文献类型：
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作者：
BLOMHOFF, R;HELGERUD, P;NORUM, KR
研究了乳糜微粒视黄酯的肝摄取。乳糜微粒是从十二指肠内注射花生油中的视黄醇的大鼠的肠淋巴中获得的。当将淋巴液静脉注射到正常大鼠中时，放射性从血液中清除，t1/2 [半衰期]约为视黄酯最初被肝脏吸收，30分钟后，肝脏中含有80-90%的注射放射性。最初，大部分放射性存在于肝细胞中，但30 min后，放射性从这些细胞中消失，并重新出现在非实质性肝细胞中。2小时后，这些细胞比肝细胞含有更多的放射性。当将淋巴液注射到维生素A缺乏大鼠或给予维甲酸形式维生素A的大鼠中时，血浆清除率和放射性的初始肝脏摄取与对照动物中发现的相似。然而，这些动物的非实质细胞未蓄积放射性。目前的数据表明，维生素A（乳糜微粒残留物）最初由肝细胞摄取，然后从这些细胞中释放，并主要输送到正常动物的非实质肝细胞。在缺乏维生素A的大鼠中，维生素从肝细胞转移到肝外组织。
Hepatic uptake of chylomicron retinyl ester was studied. Chylomicrons were obtained from intestinal lymph of rats that were given retinol in groundnut oil by intraduodenal injection. When lymph was injected i.v. into normal rats, the radioactivity was cleared from blood with t1/2 [half-life] .apprxeq. 10 min. Retinyl ester was taken up initially by the liver, which, after 30 min, contained 80-90% of the radioactivity injected. Initially, most of the radioactivity was in hepatocytes, but after 30 min it disappeared from these cells and reappeared in nonparenchymal liver cells. After 2 h these cells contained more radioactivity than the hepatocytes. When lymph was injected into vitamin A-deficient rats or rats given vitamin A in the form of retinoic acid, the plasma clearance and initial hepatic uptake of radioactivity were similar to that found in control animals. However, the nonparenchymal cells in these animals did not accumulate radioactivity. The current data suggest that vitamin A (in chylomicron remnants) is taken up initially by hepatocytes and then is released from these cells and delivered mainly to nonparenchymal liver cells in normal animals. In vitamin A-deficient rats, the vitamin is transferred from the hepatocytes to extrahepatic tissues.