Synthetic nanocircular RNA for controlling of gene expression.
Synthetic nanocircular RNA for controlling of gene expression.
复制标题
DOI：
10.1093/nass/nrp033
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Ito, Yoshihiro
中科院分区：
文献类型：
--
作者：
Abe, Hiroshi;Abe, Naoko;Ito, Yoshihiro
我们设计并合成了用于RNA干扰应用的哑铃形纳米环RNA，其由一个茎和两个环组成（1）。RNA哑铃被人Dicer酶特异性识别和切割，因此在细胞中转化为双链RNA，尽管这种RNA在血清中对降解具有抗性。优化结构以最大化其RNAi活性。当茎长为23个碱基对时，达到最有效的活性。与正常siRNA相比，RNAi活性因分子的形状而延长，分子是一种无休止的结构。
We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.