BETA-ADRENERGIC RECEPTORS IN THE HIPPOCAMPAL AND RETROHIPPOCAMPAL REGIONS OF RATS AND GUINEA-PIGS - AUTORADIOGRAPHIC AND IMMUNOHISTOCHEMICAL STUDIES
BETA-ADRENERGIC RECEPTORS IN THE HIPPOCAMPAL AND RETROHIPPOCAMPAL REGIONS OF RATS AND GUINEA-PIGS - AUTORADIOGRAPHIC AND IMMUNOHISTOCHEMICAL STUDIES
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DOI：
10.1002/syn.890130303
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发表时间：
1993-03-01
期刊：
影响因子：
2.3
通讯作者：
DAVIS, JN
中科院分区：
文献类型：
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作者：
BOOZE, RM;CRISOSTOMO, EA;DAVIS, JN
采用体外放射自显影技术研究了大鼠和豚鼠海马和海马后区β -肾上腺素能受体分布的物种差异。在两种动物的海马区CA1均发现了β 1受体，尽管豚鼠的受体密度明显低于大鼠。在豚鼠中，β 2-肾上腺素能受体在海马区CA1中占优势。两种动物海马区CA3的β 1-和β 2受体水平都很低。海马体后区也有明显的β受体分布。在大鼠中，耻骨下和副耻骨（II-III层）被大量标记为β - 1受体；相比之下，豚鼠在这些区域几乎没有受体。β 2受体在大鼠眼下区尤为突出。在大鼠和豚鼠身上都发现了内嗅皮质层含有β受体。利用免疫组织化学技术比较各组海马各亚区儿茶酚胺能神经支配模式和受体分布。尽管CA3区普遍缺乏β受体，但在大鼠和豚鼠海马CA3区观察到丰富的儿茶酚胺免疫反应纤维。海马-肾上腺素能受体亚型分布存在显著的物种差异，且两种动物的-肾上腺素能受体分布与海马肾上腺素能神经支配模式不一致。
Species differences in the distribution of beta-adrenergic receptors in the hippocampal and retrohippocampal regions of rats and guinea pigs were examined using in vitro autoradiographic techniques. Beta1-receptors were found in the hippocampal area CA1 of both species, although guinea pigs had significantly lower receptor densities in comparison to rats. In guinea pigs, beta2-adrenergic receptors were predominant in hippocampal area CA1. Hippocampal area CA3 had very low levels of beta1- and beta2-receptors in both species. The retrohippocampal area was also found to have a distinct topographic distribution of beta-receptors. In rats, the subiculum and parasubiculum (layers II-III) were heavily labeled for beta1-receptors; in contrast, guinea pigs had few receptors in these regions. Beta2-receptors were particularly prominent in the parasubicular region in rats. The entorhinal cortex laminae was found to contain beta-receptors in both rats and guinea pigs. Immunohistochemical techniques were used to compare the pattern of catecholaminergic innervation with the receptor distribution within each hippocampal subregion. Despite the general lack of beta-receptors in area CA3, abundant catecholamine immunoreactive fibers were observed in CA3 of rat and guinea pig hippocampus. Significant species differences were found in the distribution of hippocampal beta-adrenergic receptor subtypes, and moreover, in both species the distribution of beta-adrenergic receptors did not coincide with the pattern of hippocampal adrenergic innervation.