Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune
Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune
复制标题
外源海藻糖增强葡萄汉逊孢菌对塔宾曲霉和公青霉引起的葡萄浆果腐病的生防效果
DOI：
10.1002/jsfa.8998
复制
发表时间：
2018-09-01
影响因子：
4.1
通讯作者：
Kwaw, Emmanuel
中科院分区：
文献类型：
--
作者：
Apaliya, Maurice Tibiru;Zhang, Hongyin;Kwaw, Emmanuel
背景：化学农药主要用于防治水果和蔬菜的采前和采后病害。然而，公众对这些化学杀虫剂残留物可能引起的人类和环境健康问题表示强烈关注。因此，由于其对人类和动物的安全性，生物防治往往是首选的。使用的微生物拮抗剂经常会遇到不同的气候条件，这会影响它们的药效。结果：用2.0%海藻糖对葡萄腐烂病的发生和病斑直径有显著的抑制作用，而不影响4℃和20℃贮藏葡萄的感官品质；2%海藻糖对葡萄的种群动态有显著的影响(P<；0.05)。体外孢子萌发试验表明，图宾木霉和白杨的抑制率分别为85.6%和87.0%。扫描电子显微镜结果表明，未处理和使用前用2%w/v海藻糖预处理均能抑制真菌菌丝生长和葡萄腐烂的发生。结论：紫云英对葡萄腐烂病有较好的生防效果。研究结果表明，海藻糖具有潜在的应用前景。(三)2018年化学工业学会
BACKGROUND: Primarily, chemical pesticides are commonly used to control preharvest and postharvest diseases of fruits and vegetables. However, there is strong public concern regarding the human and environmental health problems that might emanate from the residues of these chemical pesticides. As a result, biocontrol is often preferred due to its safety for humans and animals. The microbial antagonists employed often encounter variable climatic conditions, which affect their efficacy. In this study, the biocontrol efficacy of Hanseniaspora uvarum enhanced with trehalose against Aspergillus tubingensis and Penicillium commune in grapes was investigated.RESULTS: H. uvarum Y3 pretreated with 2.0% w/v trehalose in nutrient yeast dextrose broth (NYDB) before used significantly inhibited the incidence of decay and lesion diameter without affecting the sensory qualities of the grapes stored at either 4 degrees C or 20 degrees C. There was also a significant (P < 0.05) increase in the population dynamics of H. uvarum that was pretreated with 2% trehalose compared to that of H. uvarum alone. The in vitro assay on spore germination revealed an inhibition of A. tubingensis and P. commune by 85.6% and 87.0% respectively. Scanning electron microscopy results showed that both untreated H. uvarum and H. uvarum pre-treated with the 2% w/v trehalose before use inhibited fungal mycelium and development of grape rot.CONCLUSION: The biocontrol efficacy of H. uvarum was enhanced against grape rot caused by A. tubingensis and P. commune. The findings indicate the potential applicability of trehalose in the enhancement of H. uvarum. (C) 2018 Society of Chemical Industry