Interleukin-1β expression in human gastric carcinoma with Epstein-Barr virus infection
Interleukin-1β expression in human gastric carcinoma with Epstein-Barr virus infection
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DOI：
10.1128/jvi.76.13.6825-6831.2002
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发表时间：
2002-07-01
影响因子：
5.4
通讯作者：
Fukayama, M
中科院分区：
文献类型：
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作者：
Chong, JM;Sakuma, K;Fukayama, M
KT肿瘤是人类eb病毒（EBV）相关胃癌（EBVaGC）的可移植株，在严重联合免疫缺陷（SCID）小鼠中建立，可以在不受浸润淋巴细胞干扰的情况下研究EBVaGC的细胞因子表达。作为EBVaGC高密度寡核苷酸阵列（GeneChip）分析的一部分，白细胞介素-1 β (il -1 β)基因是唯一在KT肿瘤细胞中表达明显高于ebv阴性GC肿瘤株的细胞因子基因。结果经Northern blotting、Western blotting和酶联免疫吸附试验证实。此外，通过原位杂交，我们在手术切除的EBVaGC的癌细胞中发现了il -1 β mRNA的阳性信号，而在ebv阴性的GC中则没有。在体外，il -1 β增加了GC细胞系TMK1的细胞生长。因此，il -1 β可能在EBVaGC中作为一种自分泌生长因子。
The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1beta (IL-1beta) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1beta mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1beta increased the cell growth of a GC cell line, TMK1. Thus, IL-1beta may act as an autocrine growth factor in EBVaGC.