NUCLEOTIDE-SEQUENCE OF RNA-1, THE LARGEST GENOMIC SEGMENT OF RICE STRIPE VIRUS, THE PROTOTYPE OF THE TENUIVIRUSES
NUCLEOTIDE-SEQUENCE OF RNA-1, THE LARGEST GENOMIC SEGMENT OF RICE STRIPE VIRUS, THE PROTOTYPE OF THE TENUIVIRUSES
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DOI：
10.1099/0022-1317-75-12-3569
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发表时间：
1994-12-01
影响因子：
3.8
通讯作者：
ISHIHAMA, A
中科院分区：
文献类型：
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作者：
TORIYAMA, S;TAKAHASHI, M;ISHIHAMA, A
利用两组重叠的cDNA克隆，测定了水稻条纹病毒（RSV）基因组最大片段RNA1的全核苷酸序列。RNA片段1包含8970个核苷酸，并且在病毒互补序列上具有编码2919个氨基酸的蛋白质的单个长开放阅读框，估计M（r）为336 860。推定蛋白的氨基酸序列比较表明与布尼亚病毒科的白蛉病毒属的L蛋白具有很强的同源性（在约1500个残基上30%的氨基酸同一性），但与布尼亚病毒科的其他成员没有可检测到的相似性。然而，与塔卡里布沙粒病毒的L蛋白检测到弱相似性。高度同源的序列结构域包括推定的RNA依赖性RNA聚合酶的保守基序。这里提供的数据，沿着与以前的工作清楚地显示RSV和布尼亚病毒科的白蛉病毒属成员之间在基因组组织、结构和表达方面的显著相似性。综上所述，我们建议应将纤细病毒属归入布尼亚病毒科纤细病毒属。
The complete nucleotide sequence of RNA 1, the largest genomic segment of rice stripe virus (RSV), was determined using two sets of overlapping cDNA clones. RNA segment 1 comprises 8970 nucleotides and on the viral complementary sequence has a single long open reading frame coding for a protein of 2919 amino acids with an estimated M(r) of 336 860. Amino acid sequence comparisons of the putative protein indicated strong homology (30 % amino acid identity over about 1500 residues) with the L protein of the genus Phlebovirus of the Bunyaviridae, but no detectable similarity with other members of the Bunyaviridae. However, weak similarity was detected with the L protein of Tacaribe arenavirus. The highly homologous sequence domain includes the conserved motifs of the putative RNA-dependent RNA polymerase. The data presented here, along with previous work clearly show significant similarities in genome organization, structure and expression between RSV and members of the genus Phlebovirus of the Bunyaviridae. Taken together, we propose that tenuiviruses should be included in the Bunyaviridae under the genus Tenuivirus.