Practical implementation of adaptive optics in multiphoton microscopy
Practical implementation of adaptive optics in multiphoton microscopy
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DOI：
10.1364/oe.11.001123
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发表时间：
2003-05-19
期刊：
影响因子：
3.8
通讯作者：
Girkin, JM
中科院分区：
文献类型：
--
作者：
Marsh, PN;Burns, D;Girkin, JM
介绍了一种采用自适应光学校正的双光子显微镜。利用微机械变形镜实现了扫描激光束的波前变换。后扫描头实施产生了与Bio-Rad MRC-600扫描头兼容的紧凑模块。利用多光子荧光强度反馈的自动像差校正允许自适应光学系统扩展人工和生物折射率失配样品中可达到的成像深度。与1.3-NA，x40，尼康油浸物镜，在水中的成像深度从约3.4到46.2 μ m的扩展与由1.25 μ m的FWHM轴向点扩散函数定义的分辨率。2003年美国光学学会。
A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 mum with a resolution defined by a FWHM axial point-spread function of 1.25 mum.. 2003 Optical Society of America.