Genetic Characterization of a Novel HIV-1 Second-Generation Recombinant Form (CRF01_AE/CRF07_BC) Identified in Yan'an City, China
Genetic Characterization of a Novel HIV-1 Second-Generation Recombinant Form (CRF01_AE/CRF07_BC) Identified in Yan'an City, China
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中国延安市鉴定出的新型 HIV-1 第二代重组体 (CRF01_AE/CRF07_BC) 的遗传特征。
DOI：
10.1089/aid.2021.0058
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发表时间：
2021-04-30
影响因子：
1.5
通讯作者：
Zhou, Yan-Heng
中科院分区：
文献类型：
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作者：
Cheng, Si-Wei;Chen, Yan-Ping;Zhou, Yan-Heng
由于CRF01_AE和CRF07_BC在中国的共流行，在中国发现了越来越多的CRF01_AE和CRF07_BC之间的第二代重组体，特别是在性人群中。本研究从陕西省延安市一名男性HIV-1阳性个体（18 YA 004）中分离到一个独特的CRF01_AE/CRF07_BC重组子。近全长基因组分析表明，18 YA 004被pol、vpr、vpu和nef基因上的5个断裂点分为6个片段。CRF01_AE的3个片段（片段I、III和V）均聚在簇4a谱系内，仅在北方中国的MSM中流行。结合我们之前在延安市发现的CRF01_AE/C重组株，CRF01_AE/CRF07_BC毒株的出现进一步表明这里存在多种HIV-1基因型共存。因此，有必要继续监测高危人群中HIV-1的分子流行病学，以更好地了解该地区HIV-1的遗传复杂性和传播。
Owing to co-epidemic of CRF01_AE and CRF07_BC in China, increasing numbers of the second-generation recombinants between them have been identified especially among sexual population. In this study, we identified a unique CRF01_AE/CRF07_BC recombinants from a male HIV-1 positive individual (18YA004) infected by heterosexual contact in Yan'an city, Shaanxi province. The near full-length genome analyses showed 18YA004 was divided into six fragments by five breakpoints located in the pol, vpr, vpu, and nef gene, respectively. Three CRF01_AE segments (segments I, III, and V) were all clustered within the cluster 4a lineage, exclusively circulating among MSM in the northern China. Coupled with our previous finding of CRF01_AE/C recombinant in Yan'an city, the emergence of CRF01_AE/CRF07_BC strain further suggested coexistence of multiple HIV-1 genotypes here. Therefore, it is necessary to continue monitoring the molecular epidemiology of HIV-1 among high-risk groups to obtain a better understanding of the genetic complexity and transmission of HIV-1 in this region.