Effects of heparin and related molecules upon neutrophil aggregation and elastase release in vitro
Effects of heparin and related molecules upon neutrophil aggregation and elastase release in vitro
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DOI：
10.1038/sj.bjp.0705291
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发表时间：
2003-06-01
影响因子：
7.3
通讯作者：
Page, CP
中科院分区：
文献类型：
--
作者：
Brown, RA;Lever, R;Page, CP
1中性粒细胞衍生的弹性蛋白酶是一种与慢性阻塞性肺疾病（COPD）发病机制有关的酶。肝素可抑制弹性蛋白酶的酶活性，在此我们首次提供证据证明肝素可抑制人嗜中性粒细胞释放弹性蛋白酶。2未分级和低分子量肝素（UH和LMWH，0.01 - 1000 U ml（-1））和相应浓度（0.06 - 6000 μ g ml（-1））的非抗凝剂O-磷酸化肝素（ODH）、硫酸葡聚糖（DS）和非硫酸化聚-L-谷氨酸（PGA）对弹性蛋白酶从嗜中性粒细胞释放和聚集的影响进行了比较。抑制ODH和LMWH（P
1 Neutrophil-derived elastase is an enzyme implicated in the pathogenesis of chronic obstructive pulmonary disease ( COPD). Heparin inhibits the enzymatic activity of elastase and here we provide evidence for the first time that heparin can inhibit the release of elastase from human neutrophils.2 Unfractionated and low molecular weight heparins (UH and LMWH, 0.01 - 1000 U ml(-1)) and corresponding concentrations ( 0.06 - 6000 mug ml(-1)) of nonanticoagulant O-desulphated heparin (ODH), dextran sulphate (DS) and nonsulphated poly-L-glutamic acid ( PGA) were compared for their effects on both elastase release from and aggregation of neutrophils.3 UH, ODH and LMWH inhibited (P