Substance P-like immunoreactivity in the parietal eye visual system of the lizard Uta stansburiana.
Substance P-like immunoreactivity in the parietal eye visual system of the lizard Uta stansburiana.
复制标题
蜥蜴 Uta stansburiana 顶叶视觉系统中的 P 物质样免疫反应性。
DOI：
10.1007/bf00204784
复制
发表时间：
1982
影响因子：
3.6
通讯作者：
Reiner,A
中科院分区：
文献类型：
--
作者：
Engbretson,GA;Brecha,N;Reiner,A
我们使用免疫荧光和过氧化物酶-抗过氧化物酶技术检查了鬣蜥 Uta stansburiana 的顶叶视觉系统是否存在 P 物质样免疫反应性。在顶眼中未发现含P物质的体细胞；然而，其丛状层含有小的（直径约 1 μm）免疫反应纤维。这些纤维显然起源于顶叶之外。顶叶神经、背囊和松果体的软脑膜中也发现了免疫反应性纤维。在正常或秋水仙碱处理的动物的任何这些区域中均未观察到标记的体细胞。之前我们证明了顶叶的离心纤维系统起源于背囊中的神经元（Engbretson 等人，1981）。背囊中明显缺乏含有 P 物质的神经元，这表明顶叶中含有 P 物质的纤维不是之前观察到的离心纤维。顶叶眼中含磷纤维的来源尚不清楚。左侧内侧缰核的背外侧部接收致密物质 P 正投影。在右侧缰核中没有看到这样的突出。顶叶神经节细胞末端的同时可视化（用顺行轴突内运输的辣根过氧化物酶标记）和 P 物质样免疫荧光显示缰核免疫反应性位点与顶叶的投影场不同。因此，缰核中的 P 阳性物质末端并非起源于顶叶。顶神经横断证实了这一结论。
We examined the parietal eye visual system of the iguanid lizardUta stansburianafor the presence of substance P-like immunoreactivity by use of both immunofluorescence and peroxidase-antiperoxidase techniques. In the parietal eye no substance P-containing somata were found; however, its plexiform layer contained small (ca. 1 μm diam) immunoreactive fibers. These fibers apparently originate outside the parietal eye. Immunoreactive fibers also were found in the parietal nerve, the dorsal sac, and the leptomeninx of the pineal gland. No labeled somata were observed in any of these regions in either normal or colchicine treated animals. Previously we demonstrated that a system of centrifugal fibers to the parietal eye originates from neurons in the dorsal sac (Engbretson et al. 1981). The apparent absence of substance P-containing neurons in the dorsal sac suggests that the substance P-containing fibers in the parietal eye are not the previously observed centrifugal fibers. The source of the substance P-containing fibers in the parietal eye is unknown. The pars dorsolateralis of the left medial habenular nucleus receives a dense substance P-positive projection. No such projection was seen in the right habenula. Simultaneous visualization of the terminals of ganglion cells of the parietal eye (labeled with orthograde intraaxonally transported horseradish peroxidase) and substance P-like immunofluorescence showed that the locus of habenular immunoreactivity is distinct from the projection field of the parietal eye. Thus the substance P-positive terminals in the habenula do not originate in the parietal eye. Transection of the parietal nerve confirmed this conclusion.