Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system
Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system
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DOI：
10.1017/s0031182009990837
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发表时间：
2010-01-01
期刊：
影响因子：
2.4
通讯作者：
Clode, P. L.
中科院分区：
文献类型：
--
作者：
Borowski, H.;Thompson, R. C. A.;Clode, P. L.
隐孢子虫是一种人畜共患的原生动物寄生虫，主要影响人类和牲畜的回肠，具有引起严重肠道疾病的潜力。我们描述了C的完整生命周期。parvum在体外系统中。随着时间的推移，使用电子显微镜观察人回盲部上皮细胞系（HCT-8）的感染培养物。其他数据的形态，发展和行为特征的不同生命周期阶段，以及确定其发生时间接种后。C的许多阶段。第一次使用扫描电子显微镜观察和形态学特征。此外，寄生-宿主相互作用和C。还观察到宿主细胞上的细小病毒。对寄生虫的生物学、增殖和与宿主细胞的相互作用的更好理解将有助于开发该疾病的治疗方法。
Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages of C. parvum and their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect of C. parvum on host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease.