Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro.
Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro.
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DOI：
10.1210/jcem.78.3.8126136
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发表时间：
1994-03
期刊：
影响因子：
--
通讯作者：
I. Ryan;E. Schriock;Robert N. Taylor
中科院分区：
文献类型：
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作者：
I. Ryan;E. Schriock;Robert N. Taylor
子宫内膜异位症是妇科常见病，发病机制尚不清楚。我们建立了一个体外模型来研究正常子宫内膜和子宫内膜异位症细胞之间表型的相似性和差异。从正常子宫内膜和子宫内膜异位症植入物中分离高度纯化的上皮细胞和基质细胞培养物。形态学特征以及免疫细胞化学标记证实这些分离株为上皮细胞和基质细胞。潜在的激素反应性，建立了从两种组织类型分离的上皮细胞和基质细胞中的雌激素受体mRNA的文件。通过竞争性放射性配体结合在基质细胞中验证了这种受体蛋白的表达，揭示了可比的受体数量和解离常数。CA-125由分离自两种组织类型的上皮细胞以相似浓度选择性分泌。PRL分泌选择性地表现出孕激素刺激的基质细胞从两种组织类型。我们的研究结果表明，从正常子宫内膜和子宫内膜异位症组织中培养的高度纯化的上皮细胞和基质细胞表达与其体内对应物相同的表型和功能标志物。这些培养物提供了有用的模型，以确定有助于这种疾病的发病机制的胰腺炎特异性细胞产物。
Endometriosis is a common gynecological disorder of unclear pathogenesis. We have established an in vitro model to investigate phenotypic similarities and differences between normal endometrial and endometriosis cells. Highly purified cultures of epithelial and stromal cells were isolated from normal endometrium and endometriosis implants. Morphological features as well as immunocytochemical markers confirm these isolates as epithelial and stromal cells. Potential hormone responsiveness was established by the documentation of estrogen receptor mRNA in epithelial and stromal cells isolated from both tissue types. Expression of this receptor protein was verified in stromal cells by competitive radioligand binding, revealing comparable receptor numbers and dissociation constants. CA-125 is selectively secreted in similar concentrations by epithelial cells isolated from both tissue types. PRL secretion is selectively exhibited by progestin-stimulated stromal cells from both tissue types. Our findings demonstrate that highly purified epithelial and stromal cells cultured from normal endometrial and endometriosis tissues express the same phenotypic and functional markers as their in vivo counterparts. These cultures provide useful models to identify endometriosis-specific cell products that contribute to the pathogenesis of this disorder.