Inhibition of ribonucleotide reductase by naturally occurring quinols from spores of Agaricus bisporus.
Inhibition of ribonucleotide reductase by naturally occurring quinols from spores of Agaricus bisporus.
复制标题
双孢蘑菇孢子中天然存在的醌醇对核糖核苷酸还原酶的抑制。
DOI：
10.1016/s0006-291x(84)80207-7
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Wick,MM
中科院分区：
文献类型：
--
作者：
FitzGerald,GB;Rosowsky,A;Wick,MM
γ- l-谷氨酰基-4-羟基苯是一种稳定的苯酚，高浓度存在于常见蘑菇双孢蘑菇的鳃组织中，它被证明能够选择性地抑制L1210白血病细胞的DNA合成。对分离酶和通透性L1210细胞的研究表明，该化合物抑制核糖核苷酸还原酶（RNR），但对DNA聚合酶无影响。结果表明，抑制DNA合成与该化合物抑制RNR的能力之间存在良好的相关性。引起这些抑制作用所需的谷氨酰-4-羟基苯浓度与产孢前驱期的鳃组织有生理相关性。
γ-L-glutaminyl-4-hydroxybenzene, a stable phenol found in high concentrations in the gill tissue of the common mushroom,Agaricus bisporus, was shown to be capable of selectively inhibiting DNA synthesis in L1210 leukemia cells. Studies with isolated enzymes and permeabilized L1210 cells revealed that this compound inhibits ribonucleotide reductase (RNR) but has no effect on DNA polymerase. The results indicated a good correlation between the inhibition of DNA synthesis and the ability of this compound to inhibit RNR. The concentration of glutaminyl-4-hydroxybenzene required to elicit these inhibitory effects has physiological relevance to the gill tissue during the prodromal period of sporulation.