A study of the in-column detection performance for chromatography separation
A study of the in-column detection performance for chromatography separation
复制标题
DOI：
10.1007/s10404-015-1561-9
复制
发表时间：
2015-03
影响因子：
2.8
通讯作者：
P. Leow;P. Chee;B. Patel;D. O’Hare
中科院分区：
文献类型：
--
作者：
P. Leow;P. Chee;B. Patel;D. O’Hare
本文研究了色谱分离的柱内检测性能。数值模拟研究了悬浮在填充色谱柱内的电极的检测效率。结果表明，填充在通道内的硅珠密度不会影响电极的检测能力。这些发现已经通过实验得到了进一步的验证。8列电极阵列提供柱内五种神经递质和代谢物（肾上腺素，多巴胺，多巴胺，血清素和5-HIAA）的完整分离过程痕迹。从柱内检测，更好的峰分辨率开发通过分离柱相比，传统的柱后检测。整个分离过程表明，在E5（离柱入口28.7 mm）处可以获得较好的分离（Rs> 1），而在E8（最后一个柱内电极）处可以在60 min内获得基线分离，Rs≥1.5。所提出的结果表明，柱内的填料材料不会阻碍电极的效率，但能够产生良好的基线分离检测，与传统的柱后检测相比。
This paper studies the in-column detection performance for chromatography separation. Numerical modeling was performed to investigate the detection efficiency of the electrodes that are suspended within a packed chromatography column. The results show that the density of the silica beads packed within the channel does not hinder the detection ability of the electrode. The findings have been further validated with experimental works. An 8 in-column electrode array provides the full separation progress traces of the five neurotransmitters and metabolites (adrenalin, dopamine, DOPAC, serotonin and 5-HIAA) within the column. From the in-column detection, better peak resolutions are developed though the separation column as compared to the conventional post-column detection. The whole progress of the separation shows that good separation (Rs> 1) can be obtained at E5 (28.7 mm from column inlet), whereas baseline separation could be obtained at E8 (last in-column electrode) withRs≥ 1.5 within 60 min. The presented results suggest that the packing materials within the column do not obstruct the efficiency of the electrodes but able to produce a good baseline separation detection as compared to conventional post-column detection.