Polyphasic taxonomy of Aspergillus fumigatus and related species
Polyphasic taxonomy of Aspergillus fumigatus and related species
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DOI：
10.3852/mycologia.97.6.1316
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发表时间：
2005-11-01
期刊：
影响因子：
2.8
通讯作者：
Samson, Robert A.
中科院分区：
文献类型：
--
作者：
Hong, Seung-Beom;Go, Selng-Joo;Samson, Robert A.
使用宏观、微观形态、生长温度制度和extrolite模式检查烟曲霉费森尤斯和相关菌种内的变异性。此外，使用了包括部分β-微管蛋白、钙调蛋白和肌动蛋白基因序列的DNA分析。对菌株进行详细检查，认为A. fumigatus等的研究结果表明，它们可分为四个类群，其中包括A. fumigatus sensu stricto，烟曲霉A. lentulus和两个新种。A.种内遗传变异性;严格意义上的烟曲霉（fumigatus sensu stricto）的序列差异较低，该物种23个菌株之间的部分β-微管蛋白和钙调蛋白基因的序列差异最多为两个碱基。然而，种内形态多样性的物种是高的，该物种的划分是模棱两可的。因此，β-微管蛋白和钙调蛋白基因序列可能是划分A. fumigatus sensu stricto species. A.根据β-微管蛋白、钙调蛋白和肌动蛋白基因序列，将来自临床来源、韩国土壤和海豚的分离物分为一个分离群，与A.烟曲霉的形态特征、生长温度和extrolite剖面。A. lentulus产生extrolites橙黄碱、cyclopiazonic酸（一种结构未知的二聚吲哚）、奈替托林、一些啶虫草酮、terrein和一些色酮基戊酸和色酮基戊酸。两对分离物（CBS 117194、117186和117520、117519）分别从A.烟曲霉属（Aspergillus fumigatus）和烟曲霉组（Aspergillus section Fumigati）的其他种（包括实型的Neutraltorya）被认为是两个新种。A. fumigatiaffinis spec. nov.产生extrolites auranthine、cycloechinulin、helvolic acid、nephrotorin、palitantin、pyripyripens、tryptoquivalins和tryptoquivalons，A. novofumigatus spec. nov.产生extrolites cycloechinuline、helvolic acid、nephrotorin、palitantin和terrein。
The variability within Aspergillus fumigalus Fresenius and related species was examined using macro-, micro-morphology, growth temperature regimes and extrolite patterns. In addition, DNA analyses including partial beta-tubulin, calmodulin and actin gene sequences were used. Detailed examination of strains, considered as A. fumigatus earlier, showed that they could be divided into four groups including A. fumigatus sensu stricto, A. lentulus and two new species. The intraspecific genetic variability within A. fumigatus sensu stricto was low, the sequence differences among 23 strains of the species was at most two bases in each partial beta-tubulin and calmodulin gene. However, intraspecific morphological diversity within the species was high and delineation of the species was equivocal. Therefore, beta-tubulin and calmodulin gene sequences Could be critical determinants for the delineation of the A. fumigatus sensu stricto species. A. lentulus including isolates from clinical origin, Korean soil and from a dolphin Clustered into an isolated group based on beta-tubulin, calmodulin and actin gene sequences, differing from A. fumigalus by morphological characters, growth temperature and extrolite profile. A. lentulus produces the extrolites auranthine, cyclopiazonic acid, a dimeric indole of unknown structure, neosartorin, some pyripyropens, terrein and some tryptoquivalins and tryptoquivalons. Two pair of isolates (CBS 117194, 117186 and 117520, 117519) Clustered into separate groups from A. fumigatus and the other Aspergillus section Fumigati species, including the teleomorph Neosartorya, are proposed as two new species. A. fumigatiaffinis spec. nov. produces the extrolites auranthine, cycloechinulin, helvolic acid, neosartorin, palitantin, pyripyropens, tryptoquivalins and tryptoquivalons, and A. novofumigatus spec. nov. produces the extrolites cycloechinuline, helvolic acid, neosartorin, palitantin and terrein.